DISSEMINATION EFFORTS TRIoH PROJECT

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1. Accepted Publication List
2004	2005	2006	2007
2. Attended conferences, meetings
2004	2005	2006	2007
3. Project Initiatives
2004	2005	2006	2007
4. Public Efforts
2004	2005	2006	2007
* pdf available

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1. PUBLICATIONS

2004

Witvrouw:

• Fikkert, V., Hombrouck, A., Van Remoortel, B., De Maeyer, M., Pannecouque, C., De Clercq, E., Debyser, Z. & Witvrouw, M. Multiple mutations in human immunodeficiency virus-1 integrase confer resistance to the clinical trial drug S-1360. AIDS. 18: 2019-2028. (2004)
• Witvrouw, M., Van Maele, B., Vercammen, J., Hantson, A., Engelborghs, Y., De Clercq, E., Pannecouque, C. & Debyser, Z. Novel inhibitors of HIV-1 integration. Current Drug Metabolism. 5: 291-304. (2004)

Esté:

• J Blanco, B Bosch, MT Fernández-Figueras, J Barretina, B Clotet and JA Esté. High level of coreceptor-independent HIV endocytosis induced by contacts between primary CD4 T cells. Journal of Biological Chemistry, 279(49): 51305–51314, 2004
• Pauls E, Esté JA. Interferent RNA as a tool in target validation. Drug Discovery Today, Technologies, 1(2):135-140, 2004
• J Barretina, A Bonjoch, J Blanco, A Llano, B Clotet and JA. Esté. Immunological and virological study of Enfuvirtide treated patients. AIDS 18:1673–1682, 2004
• J Blanco, J Barretina, B Clotet, JA Esté. Cell-surface expressed X4 and R5 HIV Envelopes kill coreceptor expressing CD4 T cells by a common gp41-dependent mechanism. Journal of Leucocyte Biology, 76(4):804-11.
• Menéndez-Arias L, Esté JA. HIV-resistance to inhibitors of viral entry. Current Pharmaceutical Design , 10(15):1845-60, 2004
• Gottikh: Pinskaya M., Romanova E., Volkov E., Deprez E., Leh H., Brochon J.-C., Mouscadet J.-F., Gottikh M. (2004) HIV-1 integrase complexes with DNA dissociate in the presence of short oligonucleotides conjugated to acridine. Biochemistry, 43 , 8735- 87 43

Darlix:

• H. BELTZ, E. PIEMONT, E. SCHAUB, D. FICHEUX, B. ROQUES, J. L. DARLIX, & Y. MELY. Role of the Structure of the top half of HIV-1 cTAR DNA on the nucleic acid destabilizing activity of the Nucleocapsid Protein NCp7. J. Mol. Biol., 2004, 338, 711-23.
• C. EGELE, E. SCHAUB, N. RAMALANJAONA, E. PIEMONT, D. FICHEUX, B. ROQUES, J. L. DARLIX, & Y. MELY. HIV-1 nucleocapsid protein binds to the viral initiation DNA sequences and chaperones their kissing interactions. J. Mol. Biol., 2004, 342, 453-466.
• Y. MELY, J. AZOULAY, H. BELTZ, J.P. CLAMME, S. BERNACCHI, D. FICHEUX, B.P. ROQUES & J.L. DARLIX. Investigation by two-photon fluorescence correlation spectroscopy of the interaction of the nucleocapsid protein of HIV-1 with hairpin loop DNA sequences. Proc. SPIE Vol. 5463, 2004, p. 105-117, Femtosecond Laser Applications in Biology; Sigrid Avrillier, Jean-Michel Tualle; Eds.
• Leblanc P, Baas D, Darlix JL. Analysis of the interactions between HIV-1 and the cellular prion protein in a human cell line. J Mol Biol. 2004; 337(4):1035-51.
• Muriaux, D., Darlix, JL. and Cimarelli, A. Targeting HIV-1 budding. Curr. Pharmacol. Des. 2004; 10(30):3725-39
• Bampi C, Jacquenet S, Lener D, Decimo D, Darlix JL. The chaperoning and assistance roles of the HIV-1 nucleocapsid protein in proviral DNA synthesis and maintenance. Curr HIV Res. 2004; 2(1):79-92.

Debyser :

• Goedele Maertens, Jo Vercammen, Zeger Debyser and Yves Engelborghs. Measuring protein-protein interactions inside living cells using single color fluorescence correlation spectroscopy: Applied to human immunodeficiency virus type 1 integrase and LEDGF/p75. FASEB, 2005 Jun; 19(8):1039-41.

Chimirri :

• Ferro S, Rao A, Zappala’ M, Chimirri A, Barreca ML, Mitvrouw M, Monforte P. Synthesis of New Potential HIV-1 Integrase Inhibitors, Heterocycles, 63, 2727-2734, 2004
• Barreca ML, Rao A.; De Luca L, Zappalà M, Gurnari C, Monforte P, De Clercq E, Van Maele B, Debyser Z, Witvrouw M, Briggs J M, Chimirri A. "Efficient 3D Database Screening for Novel HIV-1 IN Inhibitors". J Chem Inf Comput Sci, 44 (4), 1450-1455 (2004).
• Rao A, Chimirri A., Ferro S, Monforte AM, Monforte P, Zappala M.. Microwave-assisted synthesis of benzimidazole and thiazolidinone derivatives as HIV-1 RT inhibitors. ARKIVOC. pp. 147 (2004)

Dietrich :

• Königs C, O´Connor KH, Rowley MJ, Irving, JA, Wijeyewickrema L, Pustowka A, Dietrich U, Mackay IR. Requirement of multiple phage displayed peptide libraries for optimal mapping of a conformational antibody epitope on CCR5. J Immunology and Cell Biology, under revision.

 

Howarth:

• P. Pádár, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2005): An intramolecular 1,3-dipolar cycloaddition reaction towards the synthesis of chiral azetidine nucleoside analogues: the D-gluco case. Nucleosides Nucleotides Nucleic Acids24, accepted for publication.
• M. Hornyák, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2005): Investigation of the intramolecular 1,3-dipolar cycloaddition reactions of unsaturated nitrones in ionic liquids. Nucleosides Nucleotides Nucleic Acids24, accepted for publication.
• K. F. Adebambo, N. M. Howarth and G. M. Rosair (2005): Benzyl 2-amino-6-chloro-9H-purine-9-carboxylate. Acta Crystallographica Section E61, accepted for publication.

Kovacs :

• G. Ferenc, Z. Kele and L. Kovács (2005): A method for determination of N 9/N 7 alkylguanine regioisomers by ESI-MS/MS. Rapid Commun. Mass Spectrom., 19, 236-240
• G. Ferenc, P. Forgó, Z. Kele and L. Kovács (2005): A facile and regioselective synthesis of 9-alkylguanine derivatives. Collect. Czech. Chem. Commun., 70, in press.

 

Maga:

• Paolucci S, Baldanti F, Maga G, Cancio R, Zazzi M, Zavattoni M, Chiesa A, Spadari S, Gerna G. (2004) Gln145Met/Leu changes in human immunodeficiency virus type 1 reverse transcriptase confer resistance to nucleoside and nonnucleoside analogs and impair virus replication. Antimicrob Agent Chemother.;48(12):4611-7.
• Locatelli GA, Cancio R, Spadari S, Maga G. (2004) HIV-1 reverse transcriptase inhibitors: current issues and future perspectives. Curr Drug Metab.;5(4):283-90.
• Cancio R, Spadari S, Maga G. (2004) Vif is an auxiliary factor of the HIV-1 reverse transcriptase and facilitates abasic site bypass. Biochem J.;383(Pt. 3):475-82.
• Locatelli GA , Campiani G, Cancio R, Morelli E, Ramunno A, Gemma S, Hubscher U, Spadari S, Maga G. (2004) Effects of drug resistance mutations L100I and V106A on the binding of pyrrolobenzoxazepinone nonnucleoside inhibitors to the human immunodeficiency virus type 1 reverse transcriptase catalytic complex. Antimicrob Agents Chemother.;48(5):1570-80.

Mély:

• H. BELTZ, E. PIEMONT, E. SCHAUB, D. FICHEUX, B. ROQUES, J. L. DARLIX, & Y. MELY. Role of the Structure of the top half of HIV-1 cTAR DNA on the nucleic acid destabilizing activity of the Nucleocapsid Protein NCp7. J. Mol. Biol., 2004, 338, 711-23.
• R.H. STOTE, E. KELLENBERGER, H. MULLER, E. BOMBARDA, B. ROQUES, B. KIEFFER, & Y. MELY. Structure of the His44Ala single point mutant of the distal finger motif of HIV-1 nucleocapsid protein: a Combined NMR, Molecular Dynamics Simulation and Fluorescence Study. Biochemistry, 2004, 43, 7687-7697.
• C. EGELE, E. SCHAUB, N. RAMALANJAONA, E. PIEMONT, D. FICHEUX, B. ROQUES, J. L. DARLIX, & Y. MELY. HIV-1 nucleocapsid protein binds to the viral initiation DNA sequences and chaperones their kissing interactions. J. Mol. Biol., 2004, 342, 453-466.
• V. SHYNKAR, A.S. KLYMCHENKO, E. PIEMONT, A.P. DEMCHENKO & Y. MELY. Dynamics of intermolecular hydrogen bonds in the excited states of 4’-dialkylamino-3-hydroxyflavones. On the pathway to ideal fluorescent hydrogen bonding sensor. J. Phys. Chem. A, 2004, 108, 8151-8159.
• A.S. KLYMCHENKO, & Y. MELY. 7-(2-Methoxycarbonylvinyl)-3-hydroxychromones: new dyes with red shifted dual emission. Tetrahedron Lett., 2004, 45, 8391-8394.
• V.V. SHYNKAR, A.S. KLYMCHENKO, Y. MELY, G. DUPORTAIL, & V.G. PIVOVARENKO. Anion formation of 4’-dimethylamino-3-hydroxyflavone in phosphatidylglycerol vesicles induced by HEPES buffer: a steady-state and time-resolved fluorescence investigation. J. Phys. Chem B, 2004,108, 18750-18755.
• Y. MELY, J. AZOULAY, H. BELTZ, J.P. CLAMME, S. BERNACCHI, D. FICHEUX, B.P. ROQUES & J.L. DARLIX. Investigation by two-photon fluorescence correlation spectroscopy of the interaction of the nucleocapsid protein of HIV-1 with hairpin loop DNA sequences. Proc. SPIE Vol. 5463, 2004, p. 105-117, Femtosecond Laser Applications in Biology; Sigrid Avrillier, Jean-Michel Tualle; Eds.

Mouscadet:

• Pinskaya, M., Romanova, E., Volkov, E., Deprez, E., Leh, H., Brochon, J.C., Mouscadet, J.F., and Gottikh, M. (2004). Biochemistry 43, 8735-8743

Robina:

• Inmaculada Robina, Antonio J. Moreno-Vargas, Ana T. Carmona and Pierre Vogel, Glycosidase Inhibitors as potential HIV Entry Inhibitors? Current Drug Metabolism , 2004, 5, 329-361

Villalain:

• M. R. Moreno, R. Pascual y J. Villalaín. Identification of membrane-active regions of the HIV-1 envelope glycoprotein gp41 using a 15-mer gp41-peptide scan. BIOCHIMICA ET BIOPHYSICA ACTA 1661:97-105 (2004)
• Jaime Guillén, Ana J. Pérez-Berná, Miguel R. Moreno y José Villalaín. Identification of the membrane-active regions of the SARS-CoV spike membrane glycoprotein using a 16/18-mer peptide scan. Implications on the viral fusion mechanism. J. VIROLOGY 79:1743-1752 (2005)
• Roberto Pascual, Miguel R. Moreno y José Villalaín. A peptide pertaining to the loop-segment of HIV gp41 binds and interacts with model biomembranes. Implications on the fusion mechanism. J. VIROLOGY 79:0000-0000 (2005)

 

 

2005

Witvrouw

• Witvrouw, M., Fikkert, V., Hantson, A., Pannecouque, C., O'Keefe, B.R., McMahon, J., Stamatatos, L., De Clercq, E. & Bolmstedt, A. Resistance of Human Immunodeficiency Virus Type 1 to the High-Mannose Binding Agents Cyanovirin N and Concanavalin A J. Virol. 79: 7777-7784 (2005).

Witvrouw & Debyser:

• Hantson A , Fikkert V , Van Remoortel B , Pannecouque C , Cherepanov P , Matthews B , Holan G , De Clercq E , Vandamme AM , Debyser Z , Witvrouw M. Mutations in both env and gag genes are required for HIV-1 resistance to the polysulfonic dendrimer SPL2923, as corroborated by chimeric virus technology. Antivir Chem Chemother. 2005;16(4):253-66.
• Witvrouw M, Fikkert V, Vercammen J, Van Maele B, Engelborghs Y, Debyzer Z. Identification of Authentic Inhibitors of HIV integration. Curr. Med. Chem. Anti-infective agents, 2005, 4 153-165

Esté:

• Blanco J, Clotet-Codina I, Bosch B, Armand-Ugon M, Clotet B, Este JA. 2005. Multiparametric assay to screen and dissect the mode of action of anti-human immunodeficiency virus envelope drugs. Antimicrob Agents Chemother 49:3926-3929.
• Bosch, B., Clotet-Codina, J. I.Blanco, E. Pauls, G. Coma, S. Cedeño, F. Mitjans, A. Llano, M. Bofill, B. Clotet, J. Piulats, and J. A. Este. 2006. Inhibition of human immunodeficiency virus type 1 infection in macrophages by an alpha-v integrin blocking antibody. Antiviral Res in press.
• Colobran, R., P. Adreani, Y. Ashhab, A. Llano, J. A. Este, O. Dominguez, R. Pujol-Borrell, and M. Juan. 2005. Multiple products derived from two CCL4 loci: high incidence of a new polymorphism in HIV+ patients. J Immunol 174:5655-5664.
• Fernàndez, G., A. Llano, M. Esgleas, B. Clotet, J. A. Esté, and M. A. Martínez. 2006. Purifying selection of CCR5-tropic HIV-1 variants in AIDS subjects that have developed syncytium-inducing CXCR4-tropic viruses. Journal of General Virology in press.
• Gimenez-Barcons, M., M. Ribera, A. Llano, B. Clotet, J. A. Este, and M. A. Martinez. 2005. Analysis of chemokine and cytokine expression in patients with HIV and GB virus type C coinfection. Clin Infect Dis 40:1342-1349.
• Llano, A., and J. A. Este. 2005. Chemokines and other cytokines in HIV-1 infection. Inmunología 24:246-260.

Esté & Darlix

• Bosch, B., J. Blanco, I. Clotet-Codina, E. Pauls, M. Armand-Ugon, B. Grigorov, D. Muriaux, B. Clotet, J. Darlix, and J. A. Este. 2005. Inhibition of coreceptor independent cell-to-cell HIV-1 transmission by a CD4-IgG2 fusion protein. Antimicrob Agents Chemother 49:4296-4304.

Esté, Vilarrasa & Witvrouw:

• Moncunill G, Negredo E, Bosch L, Vilarrasa J, Witvrouw M, Llano A, Clotet B, Este JA. Evaluation of the anti-HIV activity of statins. AIDS. 2005 Oct 14;19(15):1697-700.

Esté & Massa:

• Armand-Ugon, M., I. Clotet-Codina, C. Tintori, F. Manetti, B. Clotet, M. Botta, and J. A. Este. 2005. The anti-HIV activity of ADS-J1 targets the HIV-1 gp120. Virology 343:141-149.
• Manetti, F.; Tintori, C.; Armand-Ugon, M.; Clotet-Codina, I.; Massa, S.; Ragno, R.; Este, J. A.; Botta, M. A Combination of Molecular Dynamics and Docking Calculations to Explore the Binding Mode of ADS-J1, a Polyanionic Compound Endowed with Anti-HIV-1 Activity. J. Chem. Inf. Model. 2006,46:1344-1351.
• M. ARMAND-UGON, I. CLOTET, C. TINTORI, F. MANETTI, B. CLOTET, M. BOTTA, J. A. ESTÉ. Homology modeling of HIV-1 gp120 and docking of molecule on its surface agree with experimental data. ANTIVIRAL RESEARCH 2005, 65 ,A29

Esté, Massa & Maga

• Manetti, F.; Esté, J. A.; Clotet-Codina, I.; Armand-Ugón, M.; Maga, G.; Crespan, E.; Cancio, R.; Mugnaini, C.; Bernardini, C.; Togninelli, A.; Carmi, C.; Alongi, M.; Petricci, E.; Massa, S.; Corelli, F.; Botta, M. Parallel Solution-Phase and Microwave-Assisted Synthesis of New S-DABO Derivatives Endowed with Subnanomolar Anti-HIV-1 Activity” J. Med. Chem. 2005, 48, 8000 -8008.

Darlix:

• J. AZOULAY, S. BERNACCHI, H. BELTZ, J.P. CLAMME, E. PIEMONT, E. SCHAUB, D. FICHEUX, B. ROQUES, J.L. DARLIX & Y. MELY. Time-resolved fluorescence and two photon FCS investigation of the interaction of HIV-1 nucleocapsid protein with hairpin loop oligonucleotides. Springer Verlag Series in Fluorescence, vol. 3, Fluorescence spectroscopy in biology: advanced methods and their applications to membranes, proteins, DNA and cells. Springer-Verlag Berlin-Heidelberg-New-York, 2005, 180-197.
• Ivanyi-Nagy R, Davidovic L, Khandjian EW, Darlix JL Disordered RNA chaperone proteins: from functions to disease. C ell Mol Life Sci. 2005 62(13):1409-17.
• Herbreteau CH, Weill L, Decimo D, Prevot D, Darlix JL, Sargueil B, Ohlmann T. HIV-2 genomic RNA contains a novel type of IRES located downstream of its initiation codon. Nat Struct Mol Biol. 2005 Oct 23.
• Jacquenet S, Decimo D, Muriaux D, Darlix JL. Dual effect of the SR proteins ASF/SF2, SC35 and 9G8 on HIV-1 RNA splicing and virion production. Retrovirology. 2005 May 22;2(1):33.

Vilarrasa :

• M Terrazas, X Ariza, and J Vilarrasa, “[N,1-15N2]-2’-Deoxyadenosines”, Org. Lett. 7, 2477, 2005.
• M Terrazas, X Ariza and J Vilarrasa, “Advantages of the Ns Group in the Reactions of N1-SO2R Inosines with Benzylamine and with 15NH3”, Tetrahedron Lett. 46, 5127, 2005.
• M. Terrazas, X. Ariza, J. Farràs, and J. Vilarrasa “A Novel Nucleophilic Approach to 1-Alkyladenosines. A Two-Step Synthesis of [1-15N]-Adenosine from Inosine”, JCS-Chem. Commun. 3968, 2005.

Debyser :

• van Griensven J, De Clercq E, Debyser Z. Hematopoietic stem cell-based gene therapy against HIV infection: promises and caveats. AIDS Rev. 2005 Jan-Mar;7(1):44-55.
• Van Maele B, Debyser Z. HIV-1 integration: an interplay between HIV-1 integrase, cellular and viral proteins. AIDS Rev. 2005 Jan-Mar;7(1):26-43.
• Van Maele, B., Van Eylen, L., Pluymers, W., and Debyser Z. Evaluation of the activity of HIV-1 integrase over-expressed in eukaryotic cells. Biochemical and Biophysical Research Communications, 327: 261-267 (2005).
• De Rijck, J, Van Maele, B, Debyser, Z. Positional effects of the central DNA flap in HIV-1-derived lentiviral vectors Biochem Biophys Res Commun. 25;328(4):987-94. (2005).
• Maertens, G, Vercammen, J., Debyser, Z., and Engelborghs, Y. Measuring protein-protein interactions inside living cells using single color fluorescence correlation spectroscopy. Application to human immunodeficiency virus type 1 integrase and LEDGF/p75. FASEB J. 19(8):1039-41 (2005).

Debyser & Witvrouw:

• Vandekerckhove L, Christ F, Van Maele B, De Rijck J, Gijsbers R, Van den Haute C, Witvrouw M, Debyser Z. Transient and Stable Knockdown of the Integrase Cofactor LEDGF/p75 Reveals Its Role in the Replication Cycle of Human Immunodeficiency Virus. J Virol. 2006 Feb;80(4):1886-96.

Debyser & Benarous:

• Busschots K, Vercammen J, Emiliani S, Benarous R, Engelborghs Y, Christ F, Debyser Z. The interaction of LEDGF/p75 with integrase is lentivirus-specific and promotes DNA binding. J Biol Chem. 2005 May 6;280(18):17841-7.

Benarous, Debyser & Witvrouw:

• Emiliani S, Mousnier A, Busschots K, Maroun M, Van Maele B, Tempe D, Vandekerckhove L, Moisant F, Ben-Slama L, Witvrouw M, Christ F, Rain JC, Dargemont C, Debyser Z, Benarous R. Integrase mutants defective for interaction with LEDGF/p75 are impaired in chromosome tethering and HIV-1 replication. J Biol Chem. 280(27):25517-23 (2005).

Chimirri :

• De Luca L, Vistoli G, Pedretti A, Barreca ML, Chimirri A. Molecular dynamics studies of the full-length integrase-DNA complex. Biochem Biophys Res Commun. 2005;336(4):1010-6.
• M. L. Barreca, L. De Luca, S. Ferro, A. Rao, A-M Monforte, and A. Chimirri . Computational and synthetic approaches for the discovery of HIV-1 integrase inhibitors, Arkivoc, in press.

Chimirri, Witvrouw & Debyser:

• Barreca ML, Ferro S, Rao A, De Luca L, Zappala M, Monforte AM, Debyser Z, Witvrouw M, Chimirri A. Pharmacophore-based design of HIV-1 integrase strand-transfer inhibitors. J Med Chem. 2005 Nov 3;48(22):7084-8.

Chimirri, Witvrouw & Debyser:

• Barreca ML, Ferro S, Rao A, De Luca L, Zappala M, Monforte AM, Debyser Z, Witvrouw M, Chimirri A. Pharmacophore-based design of HIV-1 integrase strand-transfer inhibitors. J Med Chem. 2005 Nov 3;48(22):7084-8.

Dietrich :

• Königs C, O´Connor KH, Rowley MJ, Irving, JA, Wijeyewickrema L, Pustowka A, Dietrich U, Mackay IR. Requirement of multiple phage displayed peptide libraries for optimal mapping of a conformational antibody epitope on CCR5. J Immunol Meth, 2005, 299:21-35.
• Dervillez X, Hüther A, Schuhmacher J, Griesinger C, Cohen J, von Laer D, Dietrich U. Stable expression of soluble therapeutic peptides in eukaryotic cells by multimerisation: application to the HIV-1 fusion inhibitory peptide C46. ChemMedChem, in press.
• Sticht J, Humbert M, Findlow S, Bodem J, Müller B, Dietrich U, Werner J, Kräusslich HG. A peptide inhibitor of HIV-1 assembly in vitro. Nature Struct Mol Biol, 2005, 12 (8), 671-677.
• Schreiber A, Humbert M, Benz A, Dietrich U. 3D-Epitope Explorer (3DEX): Localization of conformational epitopes within three dimensional structures of proteins. J Comp Chem 2005, 26 (9),879-887.

Divita & Restle:

• Depollier, J., Hourdou, M.-L., Aldrian-Herrada, G., Rothwell, P., Restle, T. and Divita, G., (2005) Insight into the mechanism of a peptide inhibitor of HIV reverse transcriptase dimerization. Biochemistry, 44, 1909-1918.

Engels :

• A. Zivkovic and J.W. Engels, RNA recognition by Fluor-aromatic substituted benzenes and benzimidazoles, Nucleosides, Nucleotides and Nucleic Acids 24, 1023-1027, 2005
• A.E. Klöpffer and J.W.Engels, Synthesis and purification of fluorinated benzimidazole and benzene nucleoside 5´-triphosphates, Nucleosides, Nucleotides and Nucleic Acids, 24, 651-654 2005

Gottikh :

• J. Agapkina, T. Prikazchikova, M. Smolov, M. Gottikh. Structure and functions of integrase HIV-1. Progresses in Biological Chemistry, 2005, 45, 87-122. (Russian)

Howarth:

• K. F. Adebambo, N. M. Howarth and G. M. Rosair (2005). Benzyl 2-amino-6-chloro-9H-purine-9-carboxylate. Acta Crystallographica Section E, 61, Part 2, o486-o488.

Kovacs :

• G. Ferenc, Z. Kele and L. Kovács (2005): A method for determination of N 9/N 7 alkylguanine regioisomers by ESI-MS/MS. Rapid Commun. Mass Spectrom., 19, 236-240
• G. Ferenc, P. Forgó, Z. Kele and L. Kovács (2005): A facile and regioselective synthesis of 9-alkylguanine derivatives. Collect. Czech. Chem. Commun., 70, 70, 85-102.
• G. Ferenc, Z. Kupihár, Z. Kele and L. Kovács (2005): A convenient method for the synthesis of oligonucleotide-cationic peptide conjugates. Nucleosides Nucleotides Nucleic Acids 24, 1059-1061.
• E. Szájli, G. Paragi and L. Kovács (2005): A study of H-bonding of 3- and 5-substituted 6-aminouracils in duplex and triplex structures. Nucleosides Nucleotides Nucleic Acids 24, 907-910.

Kovács & Howarth:

• P. Pádár, M. Hornyák, P. Forgó, Z. Kele, G. Paragi, N. M. Howarth and L. Kovács (2005): Intramolecular 1,3-dipolar cycloaddititon of unsaturated nitrones derived from methyl alpha-D-glucopyranoside. Tetrahedron, 61, 6816-6823.
• P. Pádár, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2005): An intramolecular 1,3-dipolar cycloaddition reaction towards the synthesis of chiral azetidine nucleoside analogues: the D-gluco case. Nucleosides, Nucleotides and Nucleic Acids, 24, 743-745.

Maga

• Crespan E, Locatelli GA, Cancio R, Hubscher U, Spadari S, Maga G. (2005) Drug resistance mutations in the nucleotide binding pocket of human immunodeficiency virus type 1 reverse transcriptase differentially affect the phosphorolysis-dependent primer unblocking activity in the presence of stavudine and zidovudine and its inhibition by efavirenz. Antimicrob Agents Chemother.;49(1):342-9.
• Cancio R, Silvestri R, Ragno R, Artico M, De Martino G, La Regina G, Crespan E, Zanoli S, Hubscher U, Spadari S, Maga G. High potency of indolyl aryl sulfone nonnucleoside inhibitors towards drug-resistant human immunodeficiency virus type 1 reverse transcriptase mutants is due to selective targeting of different mechanistic forms of the enzyme. Antimicrob Agents Chemother. 2005 Nov;49(11):4546-54.
• Franca F, Spadari S, Maga G. Human Immunodeficiency Virus (HIV-1) Auxiliary Protein Vif and Cellular APOBEC Deaminases: Their Roles Unveiled? J Biol Sci. 2005 Nov-Dec;5(6):855-63.

Massa :

• Radi, M.; Mugnaini, C.; Petricci, E.; Corelli, F.; Botta. M. Synthesis of reactive cytidine derivatives as building blocks for cross-linking oligonucleotides. Tetrahedron Lett. 2005, 46, 4361-4364.
• Mugnaini, C.; Petricci, E.; Corelli, F.; Botta, M. Combinatorial chemistry as a tool for targeting different stages of the replicative HIV-1 cycle” Comb. Chem. High Throughput Screen. 2005, 8, 387-401.
• Togninelli, A.; Carmi, C.; Petricci, E.; Mugnaini, C.; Massa, S.; Corelli, F.; Botta, M. Solution-phase parallel synthesis of S-DABO analogues. Tetrahedron Lett. 2006, 47, 65-67.
• HIV-Reverse Transcriptase Inhibition: Inclusion of Ligand-Induced Fit by Cross-docking Studies. Rino Ragno, Simona Frasca, Fabrizio Manetti, Antonella Brizzi,, Silvio Massa. J. Med. Chem. 2005 , 48, 200-212

Massa & Maga

• Radi M, Petricci E, Maga G, Corelli F, Botta M. Parallel solution-phase synthesis of 4-dialkylamino-2-methylsulfonyl-6-vinylpyrimidines. J Comb Chem. 2005 Jan-Feb;7(1):117-22.

Mély :

• N. BEN GAIED, N. GLASSER, H. BELTZ, P. WOLFF, R. MARQUET, A. BURGER, & Y. MELY. 8-vinyl-deoxyadenosine, an alternative fluorescent nucleoside analogue to 2-aminopurine with improved properties. Nucleic Acids Research, 2005, 33, 1031-1039.
• E. BOMBARDA, B.P. ROQUES , Y. MELY, & E. GRELL. Mechanism of zinc coordination by point-mutated structures of the distal CCHC binding motif of the HIV-1 NCp7 protein. Biochemistry, 2005, 44, 7315-7325.
• V.V. SHYNKAR, A.S. KLYMCHENKO, G. DUPORTAIL, A.P. DEMCHENKO, & Y. MELY. Two-color fluorescent probes for imaging dipole potential of cell plasma membranes. Biochem. Biophys. Acta., 2005, 1712, 128-136.
• C. EGELE, E. SCHAUB, E. PIEMONT, H. de ROCQUIGNY, & Y. MELY. Investigation by Fluorescence Correlation Spectroscopy of the Chaperoning Interactions of HIV-1 Nucleocapsid Protein with the Viral DNA Initiation Sequences. Comptes Rendus Biologies. 2005, 328, 1041-1051.

 

Mély & Darlix:

• H. BELTZ, C. CLAUSS, E. PIEMONT, D. FICHEUX, R.J. GORELICK, B. ROQUES, J.L. DARLIX, H. DE ROCQUIGNY, & Y. MELY. Structural determinants of HIV-1 nucleocapsid protein for cTAR DNA binding and destabilization. J. Mol. Biol., 2005, 348, 1113-1126.
• J. GODET, H. de ROCQUIGNY, C. RAJA, N. GLASSER, D. FICHEUX, J.L. DARLIX, & Y. MELY. During the early phase of HIV-1 DNA synthesis, nucleocapsid protein directs hybridization of the TAR complementary sequences via the ends of their double-stranded stem. J. Mol. Biol., 2006, in press.
• J. AZOULAY, S. BERNACCHI, H. BELTZ, J.P. CLAMME, E. PIEMONT, E. SCHAUB, D. FICHEUX, B. ROQUES, J.L. DARLIX & Y. MELY. Time-resolved fluorescence and two photon FCS investigation of the interaction of HIV-1 nucleocapsid protein with hairpin loop oligonucleotides. Springer Verlag Series in Fluorescence, vol. 3, Fluorescence spectroscopy in biology: advanced methods and their applications to membranes, proteins, DNA and cells. Springer-Verlag Berlin-Heidelberg-New-York, 2005 , 180-197.

Mouscadet:

• Bailly,F., Queffelec,C., Mbemba,G., Mouscadet,J.F., and Cotelle,P. (2005). Synthesis and HIV-1 integrase inhibitory activities of caffeic acid dimers derived from Salvia officinalis. Bioorg. Med. Chem. Lett. 15, 5053-5056.

Robina:

• Vera-Ayoso, Yolanda; Borrachero, Pastora; Cabrera-Escribano, Francisca; Gomez-Guillen, Manuel. Efficient synthesis of multifunctional furanoid C-glycoamino acid precursors. Tetrahedron: Asymmetry 2005, 164, 889-897.

Robina & Vogel :

• Moreno-Vargas, Ana T. Carmona, Federico Mora, Pierre Vogel and Inmaculada Robina. Stereoselective Synthesis of (2S,3 S,4R,5S)-5-Methylpyrrolidine-3,4-diol Derivatives that are Highly Selective alfa-L-Fucosidase Inhibitors. Chem. Commun. 2005, 4949 - 4951.
• Robina I, Vogel P. Synthesis of Aza-C-disaccharides (Diedeoxyimino-alditols C-Linked to Mono-saccharides- and Analogues. Synthesis 2005, 5, 675-702.

Villalain

• Jaime Guillén, Ana J. Pérez-Berná, Miguel R. Moreno y José Villalaín. Identification of the membrane-active regions of the SARS-CoV spike membrane glycoprotein using a 16/18-mer peptide scan. Implications on the viral fusion mechanism. J. VIROLOGY 79:1743-1752 (2005)
• Roberto Pascual, Miguel R. Moreno y José Villalaín. A peptide pertaining to the loop-segment of HIV gp41 binds and interacts with model biomembranes. Implications on the fusion mechanism. J. VIROLOGY 79:(8):5142-52.
• Pascual R, Contreras M, Fedorov A, Prieto M, Villalain J. Interaction of a peptide derived from the N-heptad repeat region of gp41 Env ectodomain with model membranes. Modulation of phospholipid phase behavior. Biochemistry. 2005 Nov 1;44(43):14275-88.

Vogel

• Łysek R, Schütz C, Vogel P. (1S,2S,3R,6R)-6-Aminocyclohex-4-ene-1,2,3-triol (=(-)—Conduramine B-1) Is a Selective Inhibitor of alpha-Mannosidases. Its Inhibitory Activity Is Enhanced by N-Benzylation. Helvetica Acta Vol. 88, 2788- 2811.
• Cardona F, Moreno G, Guarna F, Vogel P, Schütz C, Merino Pedro, Goti A. New Concise Total Synthesis of (+)-Lentiginosine and Some Structural Analogues. J. Org. Chem. 2005, 70, 6552-6555.
• Andriuzzi O, Gravier-Pelletier C, Vogel P, Le Merrer Y. Synthesis and glycosidase inhibitory activity of new penta-substituted C8-glycomimetics. Tetrahedron 61, 7094-7104.
• Łysek R, Schütz, Vogel P. Total asymmetric synthesis of (-)-conduramine B-1 and of its enantiomer. N-Benzyl derivatives of conduramine B-1 are beta-glucosidase inhibitors. Bioorg Med Chem Letters 15, 3071-3075.

 

Witvrouw

• Witvrouw, M., Fikkert, V., Hantson, A., Pannecouque, C., O'Keefe, B.R., McMahon, J., Stamatatos, L., De Clercq, E. & Bolmstedt, A. Resistance of Human Immunodeficiency Virus Type 1 to the High-Mannose Binding Agents Cyanovirin N and Concanavalin A J. Virol. 79: 7777-7784 (2005).
• Hantson A , Fikkert V , Van Remoortel B , Pannecouque C , Cherepanov P , Matthews B , Holan G , De Clercq E , Vandamme AM , Debyser Z , Witvrouw M. Mutations in both env and gag genes are required for HIV-1 resistance to the polysulfonic dendrimer SPL2923, as corroborated by chimeric virus technology. Antivir Chem Chemother. 2005;16(4):253-66.
• Witvrouw M, Fikkert V, Vercammen J, Van Maele B, Engelborghs Y, Debyzer Z. Identification of Authentic Inhibitors of HIV integration. Curr. Med. Chem. Anti-infective agents, 2005, 4 153-165

 

2006

Witvrouw:

• Al-Mawsawi, L.Q., Fikkert, V., Dayam, R., Witvrouw, M., Burke, T.R., Jr., Borchers, C., & Neamati, N. Discovery of a novel small molecule HIV-1 integrase inhibitor binding-site through photoaffinity labeling and mass spectrometry. Proc. Nat. Acad. Sciences, USA, 6. 27;103 (26):10080-5. (2006)

Esté:

• Pettersson S, Clotet-Codina I, Este JA, Borrell JI, Teixido J. Recent advances in combinatorial chemistry applied to development of anti-HIV drugs. Mini Rev Med Chem 2006,6:91-108.
• Pauls E, Senserrich J, Clotet B, Bofill M, Este JA. Induction of IL-6 and IL-8 by siRNA. Clin Exp Immunol 2007,147:189-196.
• Pauls E, Senserrich J, Clotet B, Este JA. Inhibition of HIV-1 replication by RNA interference of p53 expression. J Leukoc Biol 2006,80:659-667.
• Fernandez G, Llano A, Esgleas M, Clotet B, Este JA, Martinez MA. Purifying selection of CCR5-tropic human immunodeficiency virus type 1 variants in AIDS subjects that have developed syncytium-inducing, CXCR4-tropic viruses. J Gen Virol 2006,87:1285-1294.
• Coma G, Pena R, Blanco J, Rosell A, Borras FE, Este JA, et al. Treatment of monocytes with interleukin (IL)-12 plus IL-18 stimulates survival, differentiation and the production of CXC chemokine ligands (CXCL)8, CXCL9 and CXCL10. Clin Exp Immunol 2006,145:535-544.
• Bosch B, Clotet-Codina I, Blanco J, Pauls E, Coma G, Cedeno S, et al. Inhibition of human immunodeficiency virus type 1 infection in macrophages by an alpha-v integrin blocking antibody. Antiviral Res 2006,69:173-180.
• Este J. How to dismantle an immune response. Future Virology 2006,1:9-11. (Review)

Darlix:

• Leblanc P, Alais S, Porto-Carreiro I, Lehmann S, Grassi J, Raposo G, Darlix JL. Retrovirus infection strongly enhances scrapie infectivity release in cell culture. EMBO J. 2006 ;25(12):2674-85. This publication was selected by Nature Reviews Microbiology as research highlight: “Prion diseases: partners in crime” Nat Rev Microbiol 4, 1466; 2006.
• Grigorov B, Arcanger F, Roingeard P, Darlix JL, Muriaux D. Assembly of infectious HIV-1 in human epithelial and T-lymphoblastic cell lines.J Mol Biol. 2006 ;359(4):848-62. > COVER PAGE OF THIS ISSUE.
• Goujon C, Jarrosson-Wuilleme L, Bernaud J, Rigal D, Darlix JL, Cimarelli A. With a little help from a friend: increasing HIV transduction of monocyte-derived dendritic cells with virion-like particles of SIV(MAC). Gene Ther. 2006;13(12):991-4.
• Bampi C, Bibillo A, Wendeler M, Divita G, Gorelick RJ, Le Grice SF, Darlix JL. Nucleotide excision repair and template-independent addition by HIV-1 reverse transcriptase in the presence of nucleocapsid protein. J Biol Chem. 2006; 281(17):11736-43.
• Jarrosson-Wuilleme L, Goujon C, Bernaud J, Rigal D, Darlix JL, Cimarelli A. Transduction of nondividing human macrophages with gammaretrovirus-derived vectors. J Virol. 2006 ;80(3):1152-9.
• Grigorov, B., Roingeard, P., Darlix, JL., Muriaux, D. L’assemblage du HIV se fait-il à couvert ou à découvert ? Virologie 2006 10 :311-312.

Vilarrasa :

• L Bosch, J Vilarrasa. Cu 2(OTf) 2-Catalyzed and MW-Controlled Preparation of Tetrazoles from Nitriles and Organic Azides under Mild, Safe Conditions. Angew. Chem. Int. Ed., submitted Dec. 2006.

Debyser

• Van Maele B, Busschots K, Vandekerckhove L, Christ F, Debyser Z. Cellular co-factors of HIV-1 integration. Trends Biochem Sci. 2006 Feb 2. [abstract] [pdf]
• Vandekerckhove L, Christ F, Van Maele B, De Rijck J, Gijsbers R, Van den Haute C, Witvrouw M, Debyser Z. Transient and Stable Knockdown of the Integrase Cofactor LEDGF/p75 Reveals Its Role in the Replication Cycle of Human Immunodeficiency Virus. J Virol. 2006; 80(4):1886-96. [abstract] [pdf]
• De Rijck J, Vandekerckhove L, Gijsbers R, Hombrouck A, Hendrix J, Vercammen J, Engelborghs Y, Christ F, Debyser Z. Over-expression of the LEDGF/p75 Integrase Binding Domain Inhibits HIV Replication. J Virol. 2006 Epub Sep 20. [abstract] [pdf-preversion]
• De Rijck J, Debyser Z. The central DNA flap of the human immunodeficiency virus type 1 is important for viral replication. Biochem Biophys Res Commun. 2006;349(3):1100-10. [abstract] [pdf]

Benarous:

• Blot G, Lopez-Verges S, Treand C, Kubat NJ, Delcroix-Genete D, Emiliani S, Benarous R, Berlioz-Torrent C.Luman, a new partner of HIV-1 TMgp41, interferes with Tat-mediated transcription of the HIV-1 LTR. J Mol Biol. 2006 Dec 15;364(5):1034-47.
• Lopez-Verges S, Camus G, Blot G, Beauvoir R, Benarous R, Berlioz-Torrent C. Tail-interacting protein TIP47 is a connector between Gag and Env and is required for Env incorporation into HIV-1 virions. Proc Natl Acad Sci U S A. 2006 Oct 3;103(40):14947-52.
• Treand C, du Chene I, Bres V, Kiernan R, Benarous R, Benkirane M, Emiliani S. Requirement for SWI/SNF chromatin-remodeling complex in Tat-mediated activation of the HIV-1 promoter. EMBO J. 2006 Apr 19;25(8):1690-9.

Chimirri :

• ML Barreca, L. De Luca, Chimirri A. (2006). Binding mode prediction of strand transfer HIV-1 integrase inhibitors using Tn5 transposase as a plausible surrogate model for HIV-1 integrase. J. Med.Chem. vol. 49, pp. 3994-3997
• Maria Letizia Barreca, Laura De Luca, Stefania, Ferro, Angela Rao, Anna-Maria Monforte, Chimirri A. (2006). Computational and synthetic approaches for the discovery of HIV-1 integrase inhibitors. ARKIVOC. pp. 224-244

Dietrich

• Humbert M, Antoni A, Brill B, Landersz M, Rodes B, Soriano V, Wintergerst U, Knechten H, Staszewski S, von Laer D, Dittmar MT, Dietrich U. Vaccine-relevant mimotopes selected with HIV-1 neutralizing antibodies from long-term non-progressors. J Exp Med, submitted.
• Königs C, Pustowka A, Wegner V, Dervillez X, Hüther A, Klaukien V, Rowley MJ, Mackay IR, Kreuz W, Griesinger C, Dietrich U. Peptide mimotopes selected with HIV-1 neutralizing monoclonal antibodies against CCR5 represent functional HIV-1 entry motifs on CCR5. AIDS Res Hum Retrovir, under revision.
• Humbert M & Dietrich U. The role of neutralizing antibodies in HIV-infection. AIDS Reviews 2006, 8: 51-59.

Divita

• Gros E, Deshayes S, Morris MC, Aldrian-Herrada G, Depollier J, Heitz F, and Divita G. (2006) A non-covalent peptide-based strategy for protein and peptide nucleic acid transduction. Biochim Biophys Acta.1758, 384-393.
• Deshayes S, Morris MC, Divita G, Heitz F.(2006) Interactions of amphipathic carrier peptides with membrane components in relation with their ability to deliver therapeutics. J Pept Sci. 12(12):758-65
• Deshayes S, Plenat T, Charnet P, Divita G, Molle G, Heitz F. Formation of transmembrane ionic channels of primary amphipathic cell-penetrating peptides. Consequences on the mechanism of cell penetration. Biochim Biophys Acta. (2006), 1758(11):1846-51.
• Bampi C, Bibillo A, Wendeler M, Divita G, Gorelick RJ, Le Grice SF, Darlix JL. (2006) Nucleotide excision repair and template-independent addition by HIV-1 reverse transcriptase in the presence of nucleocapsid protein. J Biol Chem. 28;281(17):11736-43.
• Nguyen QN, Chavli RV, Marques JT, Conrad PG Jr, Wang D, He W, Belisle BE, Zhang A, Pastor LM, Witney FR, Morris M, Heitz F, Divita G, Williams BR, McMaster GK. (2006) Light controllable siRNAs regulate gene suppression and phenotypes in cells. Biochim Biophys Acta. 1758(3):394-403.
• Deshayes S, Morris MC, Divita G, Heitz F. (2006) Interactions of amphipathic CPPs with model membranes. Biochim Biophys Acta. 1758(3):328-35.
• Morris, MC ., Gros, E ., Aldrian-Herrada, G ., Choob, M., Archdeacon, J., Heitz, F., and Divita, G . (2007). A non-covalent peptide-based carrier for in vivo delivery of DNA mimics. Nucleic Acid Res. In press
• Crombez L, Charnet A, Morris MC, Aldrian-Herrada G, Heitz F, Divita G. A non-covalent peptide-based strategy for siRNA delivery, Biochem Soc Trans. (2007), 35, 44-47

Engels, Debyser & Witvrouw

• Adams MM, Bats JW, Nikolaus NV, Witvrouw M, Debyser Z, Engels JW. Microwave-assisted synthesis of fluoroquinolones and their nucleosides as inhibitors of HIV integrase. Coll Czech Chem Comm 71 (7): 978-990, 2006. [pdf]

Gottikh & Mouscadet

• Agapkina Julia, Smolov Maksim, Barbe Sophie, Zubin Evgenii, Zatsepin Timofei, Deprez Eric, Le Bret Marc, Mouscadet Jean-François, Gottikh Marina. PROBING OF HIV-1 INTEGRASE-DNA INTERACTIONS USING NOVEL ANALOGS OF VIRAL DNA. J Biol Chem 281(17):11530-1540.
• Elvire Guiot, Kevin Carayon, Olivier Delelis, Patrick Tauc, Françoise Simon, Marina Gottikh, Jean-François Mouscadet, Jean-Claude Brochon and Eric Deprez. Relationship between the Molecular Size of HIV-1 Integrase-DNA Complexes and Enzymatic Activity. J Biol Chem 281(32) p22707-22719.
• Maksim Smolov, Marina Gottik1, Vadim Tashlitskii, Sergei Korolev, Ilya Demidyuk, Jean-Claude Brochon, Jean-François Mouscadet & Eric Deprez. Kinetic Study of the HIV-1 DNA 3’-End Processing: Single-Turnover Property of Integrase. FEBS Lett. 273:1137-1151.

Keppler

• Goffinet C, Keppler OT. (2006). Efficient non-viral gene delivery into primary lymphocytes from mice and rats, FASEB Journal, in press e-pub. 9 January 2006.

Kovacs

• P. Merino, P. Pádár, I. Delso, M. Thirumalaikumar, T. Tejero and L. Kovács (2006): Stereoselective synthesis of pyrrolidinyl glycines from nitrones: complementarity of nucleophilic addition and 1,3-dipolar cycloaddition. Tetrahedron Lett.47, 5013-5016.

Kovacs & Howarth

• P. Pádár, A. Bokros, G. Paragi, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2006): Single diastereomers of polyhydroxylated 9-oxa-1-azabicyclo[4.2.1] nonanes from intramolecular 1,3-dipolar cycloaddition of omega-unsaturated nitrones. J. Org. Chem. 71, 8669-8672.
• M. Hornyák, Z. Kele, L. Kovács, P. Forgó and N. M. Howarth (2006): Investigation of 1,3-dipolar cycloaddition reactions of unsaturated nitrones in ionic liquids. Nucleosides Nucleotides Nucleic Acids 25, 867-970.

Maga

• avecchia A, Costi R, Artico M, Miele G, Novellino E, Bergamini A, CrespanE, Maga G, Di Santo R. Arylthiopyrrole (AThP) Derivatives as Non-Nucleoside HIV-1 ReverseTranscriptase Inhibitors: Synthesis, Structure-Activity Relationships, andDocking Studies (Part 2).ChemMedChem. 2006 Dec 11;1(12):1379-1390.
• Di Santo R, Costi R, Artico M, Miele G, Lavecchia A, Novellino E, BergaminiA, Cancio R, Maga G. Arylthiopyrrole (AThP) Derivatives as Non-Nucleoside HIV-1 Reverse Transcriptase Inhibitors: Synthesis, Structure-Activity Relationships, and Docking Studies (Part 1).ChemMedChem. 2006 Dec 11;1(12):1367-1378.
• De Martino G, La Regina G, Ragno R, Coluccia A, Bergamini A, Ciaprini C,Sinistro A, Maga G, Crespan E, Artico M, Silvestri R. Indolyl aryl sulphones as HIV-1 non-nucleoside reverse transcriptase inhibitors: synthesis, biological evaluation and binding mode studies of newderivatives at indole-2-carboxamide. Antivir Chem Chemother. 2006;17(2):59-77.
• Di Santo R, Costi R, Artico M, Ragno R, Lavecchia A, Novellino E, Gavuzzo E, La Torre F, Cirilli R, Cancio R, Maga G. Design, synthesis, biological evaluation, and molecular modeling studies of TIBO-like cyclic sulfones as non-nucleoside HIV-1 reverse transcriptaseinhibitors.ChemMedChem. 2006 Jan;1(1):82-95.
• Ragno R, Coluccia A, La Regina G, De Martino G, Piscitelli F, Lavecchia A,Novellino E, Bergamini A, Ciaprini C, Sinistro A, Maga G, Crespan E, Artico M, Silvestri R. Design, molecular modeling, synthesis, and anti-HIV-1 activity of new indolylaryl sulfones. Novel derivatives of the indole-2-carboxamide. J Med Chem. 2006 Jun 1;49(11):3172-84.
• Franca R, Spadari S, Maga G. APOBEC deaminases as cellular antiviral factors: a novel natural host defensemechanism.Med Sci Monit. 2006 May;12(5):RA92-8. Review.
• Silvestri R, Maga G Current state-of-the-art in preclinical and clinical development of novel non-nucleoside HIV-1 reverse transcriptase inhibitors (2006) Expert Opinion on Therapeutic Patents, 16(7): 939-962.

Massa

• Togninelli A, Carmi C, Petricci E, Mugnaini C, Massa S, Corelli F, Botta M. Solution-phase parallel synthesis of S-DABO analogues. Tetrah. Let 2006, 47,65- 67

Massa , Esté & Maga

• Mugnaini C, Manetti F, Este JA, Clotet-Codina I, Maga G, Cancio R, Botta M,Corelli F. Synthesis and biological investigation of S-aryl-S-DABO derivatives as HIV-1inhibitors.Bioorg Med Chem Lett. 2006 16(13):3541-4

Massa & Maga

• Botta M, Maga G, Novel 6-vinyl pyrimidine non nucleoside analogs are competitive inhibitors of HIV-1 reverse transcriptase with respect to the nucleotide substrate (draft in progress)

Mély

• Yushchenko DA, Shvadchak VV, Bilokin' MD, Klymchenko AS, Duportail G, Mely Y, Pivovarenko VG. Modulation of dual fluorescence in a 3-hydroxyquinolone dye by perturbation of its intramolecular proton transfer with solvent polarity and basicity. Photochem Photobiol Sci. 2006 Nov;5(11):1038-44.
• Klymchenko AS , Stoeckel H, Takeda K, Mély Y. Fast response ratiometric fluorescence probe to cellular transmembrane potential. J. Phys Chem B 110:13624-13632.
• M’Baye GM, Shynkar VV, Klymchenko AS, Mély Y, Duportail G. Membrane Dipole Potential as maeasured by ratiometric 3-hydrowyflavone fluorescence probes : accounting for hydration effects. J Fluor 13(1):35-42.
• Yushchenko DA, Volodymyr VS, Klymchenko AS, Duportail G, Mély Y, Psovarenko VG. 2-Aryl-3-hydroxyquinolones, a new class of dyes with solvent dependent dual emission due to excited state intramolecular proton transfer. New J Chem (2006) 30:774-781.
• Kenfack CA, Burger A, Mély Y (2006). Excited-State Properties and Transitions of Fluorescent 8-Vinyl Adenosine in DNA. J Phys Chem. B 110:26327-26336.
• Yushchenko DA, Bilokin' MD, Pyvovarenko OV, Duportail G, Mély Y, Pivovarenko VG. Synthesis and fluorescence properties of 2-aryl-3-hydroxyquinolones, a new class of dyes displaying dual fluorescence. Tetr. Lett. 47:905-908.

Mély & Darlix

• Darlix JL, Garrido JL, Morellet N, Mély Y, Hugues de Rocquigny. The multifunctional nature of the HIV-1 Nucleocapsid protein.Book chapter in ‘HIV PATHOGENESIS’ edited by the NIH and Elsevier (USA). In press.
• Godet J, de Rocquigny H, Raja C, Glasser N, Ficheux D, Darlix JL, Mely Y. During the early phase of HIV-1 DNA synthesis, nucleocapsid protein directs hybridization of the TAR complementary sequences via the ends of their double-stranded stem. J Mol Biol. 2006 ;356(5):1180-92.

Mély, Darlix, Dietrich

• Raja C, Ferner J, Dietrich U, Avilov S, Ficheux D, Darlix JL, de Rocquigny H, Schwalbe H, Mely Y. A tryptophan-rich hexapeptide inhibits nucleic acid destabilization chaperoned by the HIV-1 nucleocapsid protein. Biochemistry. 2006 ;45(30):9254-65.
• De Rocquigny H, Shvadchak V, Aviloc S, Zhi Dong C, Dietrich U, Darlic JL, Mély Y. Targeting the viral nucleocapsid protein in anti-HIV-1 therapy. MRMC in press.

Mouscadet

• Majerz-Maniecka,K., Musiol,R., Nitek,W., Oleksyn,B.J., Mouscadet,J.F., Bret,M.L., and Polanski,J. (2006). Intermolecular interactions in the crystal structures of potential HIV-1 integrase inhibitors. Bioorg. Med. Chem. Lett. 16, 1005-1009.
• Maurin C , Bailly F , Mbemba G , Mouscadet JF , Cotelle P (2006). Design, synthesis, and anti-integrase activity of catechol-DKA hybrids. Bioorg Med Chem 14(9):2978-84..
• Niedbala,H., Polanski,J., Gieleciak,R., Musiol,R., Tabak,D., Podeszwa,B., Bak,A., Palka,A., Mouscadet,J.F., Gasteiger,J., and Le,B.M. (2006). Comparative molecular surface analysis (CoMSA) for virtual combinatorial library screening of styrylquinoline HIV-1 blocking agents. Comb. Chem. High Throughput. Screen. 9, 753-770.
• Tchertanov, L. & Mouscadet, J.-F.Target Recognition by Catechols and beta-Ketoenols: Potential contribution of hydrogen bonding and Mn/Mg chelation to HIV-1 integrase  inhibition. Journal of Medicinal Chemistry. In press
• Maurin,C., Bailly,F., Mbemba,G., Mouscadet,J.F., and Cotelle,P. (2006). Design, synthesis, and anti-integrase activity of catechol-DKA hybrids. Bioorg. Med. Chem. 14, 2978-2984.
• Majerz-Maniecka,K., Musiol,R., Nitek,W., Oleksyn,B.J., Mouscadet,J.F., Le,B.M., and Polanski,J. (2006). Intermolecular interactions in the crystal structures of potential HIV-1 integrase inhibitors. Bioorg. Med. Chem. Lett. 16, 1005-1009.
• Polanski J, Niedbala H, Musio R, Podeszwa B, Tabak D, Palka A, Mencel A, Mouscadeet JF, Le Bret M. Fragment Based Approach for the Investigation of HIV-1 Integrase Inhibition. Let. Drug Design & Discovery in press.
• Su,C.X., Mouscadet,J.F., Chiang,C.C., Tsai,H.J., and Hsu,L.Y. (2006). HIV-1 integrase inhibition of biscoumarin analogues. Chem. Pharm. Bull. ( Tokyo) 54, 682-686.

Restle

• Veldhoen, S. and Restle, T., (2006) Recent developments in peptide-based cellular delivery of nucleic acids. Recent Devel. Nucleic Acids Res. 2, 201-236, TRN, ISBN: 81-7895-192-4.
• Mescalchin, A., Wünsche, W., Laufer, S., Grohmann, D., Restle, T. and Sczakiel, G., (2006) Specific binding of a bioactive hexanucleotide to HIV-1 reverse transcriptase: a novel class of small oligomeric nucleic acid drugs. NAR, 34 (19), 5631–5637.
• Veldhoen, S., Laufer, S.D.,Trampe, A. andRestle, T., (2006) Cellular delivery of small interfering RNA by a non-covalently attached cell-penetrating peptide: quantitative analysis of uptake and biological effect. NAR, 34 (22), 6561–6573.

Robina

• Carmona AT, Fialová P, Křen V, Ettrich R, Martínková L, Moreno-Vargas AJ, González C, Robina I. “Cyanodeoxy-Glycosyl derivatives as substrates for enzymatic reactions”, Eur. J. Org. Chem. 2006, 1876-1885.
• Moreno-Clavijo E, Carmona AT, Moreno AJ, Robina I.” Stereoselective synthesis of novel five-membered homoazasugars. A convenient route to all-cis- tetrasubstituted pyrrolidines”. Tetrahedron Lett., 2007, 48, 159-162.
• Molina L, Moreno-Vargas AJ, Carmona AT, Robina I. “Stereoselective Synthesis of Chiral Amino Acid Analogues of D- and L-Serine from Sugars” Synlett 2006, 1327-1330.
• Vera-Ayoso Y, Borrachero P, Cabrera-Escribano F, Gomez-Guillen M, Vogel P. “A novel approach to the synthesis of N-substituted 1-C-aminomethyl glycofuranosides.” Synlett 2006, 1, 45-48.

Veljkovic , Mouscadet & Debyser

• Veljkovic V, Mouscadet JF, Veljkovic N, Glisic S, Debyser Z. Simple criterion for selection of flavonoid compounds with anti-HIV activity. Bioorg Med Chem Lett. Bioorg Med Chem Lett. 2007 Mar 1;17(5):1226-32..

Veljkovic , Esté & Dietrich

• Veljkovic V, Veljkovic N, Esté JA, Hüther A and Dietrich U. Application of the EIIP/ISM bioinformatics concept in development of new drugs. Curr Med Chem. 2007; 14: 133-155.

Villalain

• Moreno M, Giudici M, Villalaín J. The membranotropic regions of the endo and ectodomains of the HIV gp41 envelope glycoprotein. BIOCHIM. BIOPHYS. ACTA BIOMEMBRANES 1758, 111-123 (2006).
• Pérez-Berná AJ , Moreno MR, Guillén J, Bernabeu A, Villalaín J. The membrane-active regions of the HCV E1 and E2 envelope glycoproteins. BIOCHEMISTRY 45, 3755-3768 (2006)

Vogel

• Lysek R, Vogel P. Synthesis of amino- and diaminoconduritols and their applications. Tetrahedron (2006), 62(12), 2733-2768.

Vogel & Esté :

• Lysek R, Schutz C, Favre S, O'Sullivan AC, Pillonel C, Krulle T, Jung PMJ, Clotet-Codina I, Este JA, Vogel P. Search for alpha-glucosidase inhibitors: new N-substituted valienamine and conduramine F-1 derivatives. Bioorg Med Chem 2006,14:6255-6282.

 

2007

Debyser & Witvrouw

• Hombrouck A, De Rijck J, Hendrix J, Vandekerckhove L, Voet A, De Maeyer M, Witvrouw M, Engelborghs Y, Christ F, Gijsbers R, Debyser Z (2007) Virus Evolution Reveals an Exclusive Role for LEDGF/p75 in Chromosomal Tethering of HIV. PLoS Pathog 3(3): e47. [abstract] [free full text]

Debyser, Benarous & Rain:

• Busschots K, Voet A, De Maeyer M, Rain JC, Emiliani S, Benarous R, Desender L, Debyser Z, Christ F. Identification of the LEDGF/p75 binding site in HIV-1 integrase. J mol biol (2007) 365, 1480–1492. [abstract] [pdf]

Darlix

• Goujon C, Riviere L, Jarroson-Wuilleme L, Bernaud J, Rigal D, Darlix JL, Cimarelli A. SIVSM/HIV-2 Vpx proteins promote retroviral escape froma proteasome-dependent restriction pathmay present in human dendritic cells. Retrovirology. 2007 Epub 9 Jan.
• Balvay L, Lastra ML, Sargueil B , Darlix JL, Ohlmann T. Translational control of retroviruses.NATURE REVIEWS Microbiology 2007 Feb;5(2):128-40..
• Gatignol A, Dubuisson J, Wainberg MA, Cohen EA, Darlix JL. New pa ndemics: HIV and AIDS, HCV and chronic hepatitis, Infl uenza virus and flu. Retrovirology. 2007 Feb 1;4(1):8

Chimirri & Maga

• Barreca ML, DeLuca L, Iraci N, Rao A, Ferro S, Maga G and Chimirri A Struture-based pharmacophore identification of new chemical scaffolds as non-nucleoside reverse transcriptase inhibitors (2006) J. Chem. Inf. Mod. 2007 47(2):557-62. [abstract]

Divita

• Gerbal-Chaloin S, Gondeau C, Aldrian-Herrada G, Heitz F, Gauthier-Rouviere C, Divita G. First step of the cell penetrating peptide mechanism involves Rac1 GTPase dependent actin-network remodelling. Biol Cell. 99(4):223-38.

Engels

• Nikolaus NV, Bozilovic J, Engels JW. Microwave-assisted Ribosylation of modified heterocyclic bases by Vorbrüggen method. Nucl. Nucl. 2007 in print

Keppler

• Goffinet C, Allespach I, Keppler OT. HIV-susceptible transgenic rats allow rapid preclinical testing of antiviral compounds targeting virus entry or reverse transcription. Proc Natl Acad Sci U S A. 2007 Jan 5. Reported in Nature Reviews Drug Discovery 6, 112-113 (February 2007) by Joanna Owens as Research Highlight.

Maga & Esté

• Cancio R, Mai A, Rotili D, Artico M, Sbardella G, Clotet-Codina I, Este JA, Crespan E, Zanoli S, Hubscher U, Spadari S, Maga G.Slow-, Tight-Binding HIV-1 Reverse Transcriptase Non-Nucleoside Inhibitors Highly Active against Drug-Resistant Mutants. ChemMedChem. 2007 Apr 16;2(4):445-448.

Maga, Massa & Vilarrasa :

• G Maga, M Radi, S Zanoli, F Manetti, R. Cancio, U Húbscher, S Spadari, C Falciani, M Terrazas, J Vilarrasa, M Botta. Discovery of the First Non-Nucleoside Inhibitors of HIV-1 Reverse Transcriptse Competing with the Nucleoside Substrate, Angew. Chem. Int. Ed. 46(11):1810-3.

Massa

• Radi M, Petricci E, Corelli F, Botta M. Stereoselective synthesis of N1-6-methyluridine and related 2-substituted analogues. (2007). HETEROCYCLES, In press

Mély

• M'Baye G, Klymchenko AS, Yushchenko DA, Shvadchak VV, Ozturk T, Mely Y, Duportail G. Fluorescent dyes undergoing intramolecular proton transfer with improved sensitivity to surface charge in lipid bilayers. Photochem Photobiol Sci. 2007 Jan;6(1):71-76.
• Shynkar VV, Klymchenko AS, Kunzelmann C, Duportail G, Muller CD, Demchenko AP, Fresyssinet JM, Mély Y. Fluorescent Biomembrane probe for ratiometric detection of apoptosis. J Am Chem Soc in press (2007).

Mély, Darlix, Dietrich

• De Rocquigny H, Shvadchak V, Aviloc S, Zhi Dong C, Dietrich U, Darlic JL, Mély Y. Targeting the viral nucleocapsid protein in anti-HIV-1 therapy. MRMC in press.

Veljkovic, Dietrich & Esté

• Veljkovic V, Veljkovic N, Este JA, Huther A, Dietrich U. Application of the EIIP/ISM bioinformatics concept in development of new drugs. Curr Med Chem. 2007;14(4):441-53.

 

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2. CONGRESSES, MEETINGS

2004

General conference given on novel inhibitors integrase for the 10 th anniversary of the French SIDACTION, the largest non-profit organisation dedicated to AIDS research. http://www.sidaction.org/ Summary of the conference can be found in the publication “Transversal” avril/mai/juin 2004, France. A Sidaction publication.

4 th Frederick Workshop on the Cell Biology of Viral Entry, May, 2-5, 2004, Frederick, MD, USA. http://web.ncifcrf.gov/events/viral_entry

Symposium “Peptides as drugs: problems and promise”, October, 28-29, 2004, Frankfurt, Germany http://www.georg-speyer-haus.de/intra/seminare/index.htm

CROI 2005, Presentation of the work entitle: Inhibition of coreceptor independent cell to cell HIV-1 transmission by B Bosch, J Blanco, MT Fernández-Figueras, B Clotet and JA Esté. http://www.retroconference.org

European Drug Discovery Techology. Invited Speaker. Title: Targeting Replication and Integration of HIV: European Research Initiative for Developing New Anti-HIV Agent. http://www.drugdisc.com/europe/

TRIoH Scientific Symposium: different aspects of HIV replication and integration; part of Training Program, target public are young researchers and African students. http://www.kuleuven.be/molvirgen/project/trioh/symposium.htm

18 th Int. Conference on Antiviral Research, Barcelona, 2005. J. Esté Local Organizer . 3oral/poster. http://www.georgetown.edu/research/arc/ISAR/news.html

Contribution to the European Workshop on FCS could be seen on the following webpage:http://www.zeiss.fr/C12568E80022E0FD/Contents-Frame/D812E43F07616B0BC1256EEB0029F328

16th International Round Table Nucleosides, Nucleotides and Nucleic Acids . 6 members of our Laboratory has participated this international event which is the most important one in our field. We have presented several posters at this conference. Date and venue: 12-16 September 2004, Minneapolis, Minnesota, USA: Website: http:// www.cdd.umn.edu/is3na (site no longer available). The presented posters (4) attracted some 80 interested scientists.

22nd Informal Meeting on Mass Spectrometry. 1 member of our Laboratory has participated this international event and presented 1 poster. Date and venue: 2-6 May, Tokaj, Hungary. The presented poster (1) attracted some 20 interested scientists.

 

Additional list of attended meetings, congresses, and workshops related to the project (communication format).

 

Chimirri :

XVII National Meeting of the Medicinal Chemistry Division –Italian Chemical Society, 6-10 Sept 2004, Pisa , Italy . De Luca L, Pedretti A, Vistoli G, Barreca ML, Villa L, Monforte P, Chimirri A. Sviluppo del modello DNA-integrasi dell’HIV-1: studi di docking e di dinamica molecolare , CO-24 . http://www.lbstudio.it/eventi/dcf2004/index.html

Congresso regionale della Società Chimica Italiana (SCI), 2-3 December 2004, Messina, Italy. Barreca ML, Chimirri A, Debyser Z, De Luca L, Ferro S, Monforte AM, Rao A, Witvrouw M, Zappalà M. Progettazione e Sintesi di Nuovi Inibitori dell’Integrasi dell’HIV-1.

Joint Meeting on Medicinal Chemistry, 20-23 June 2005, Vienna , Austria http://jmmc.goech.at/

20 th International Conference Heterocyclic Chemistry, July 31 – August 5 2005, Palermo, Italy http://www.20ichc.palermo.it/home.htm

Darlix :

JL DARLIX, D. MURIAUX, A. CIMARELLI. TRIoH meeting. Ecole normale supérieure de Lyon, Lyon, 7-8 mars 2004.

JL DARLIX. Promega General conference on the origins of HIV, Lyon, France. 27th May 2004.

JL DARLIX . Homologies between HIV and HCV. Faculty of Medicine, Tours, France. 3rd of June 2004

JL DARLIX. HIV replication, HAART. Institut de recherche et développement (IRD), 9th and 10th June 2004 ; Banuyls, FRANCE

JL DARLIX. D. MURIAUX. B GRIGOROV. General conference on HIV. Seminar on HIV and lentivectors for gene therapy. - Faculty of Medicine of SOFIA, BULGARIA ; 30th of June 2004

JL DARLIX. Courses on HIV to european students. Université Européenne d’été, Toulouse, FRANCE, 12th July 2004.

JL DARLIX. General conference to medical doctors and researchers coming from Europe and Africa. Université d’été des jeunes chercheurs sur le SIDA, Marseille, France. 21st, 22nd September 2004.

JL DARLIX. Courses in molecular medicine on HIV and lentivectors.

Lyon , conference and courses on HIV in Molecular Medicine. 26th November, 1st and 13 th December 2004.

D MURIAUX, B. GRIGOROV. Oral presentation and poster. 13th International Symposium on HIV & Emerging Infectious Diseases, 3-5 June 2004, Toulon, France.

D. MURIAUX, B GRIGOROV, JL DARLIX. HIV GAG TRAFFICKING. 2004 MEETING on RETROVIRUSES. Cold Spring Harbor, New York USA.

D. MURIAUX, D DECIMO, C PECHOUX, B GRIGOROV AND JL DARLIX. Role of NC in HIV GAG trafficking. Cold Spring Harbor, New York USA.

Dietrich:

HIV-1 entry meeting in Frederick/USA: poster presentation;

HIV meeting in Baltimore/USA: poster presentation

Engels :

Round Table on Nucleosides, Nucleotides and Nucleic Acids, Minneapolis, Minnesota, 12-16.9. 2004

Poster on Fluorobenzimidazole Nucleosides

website: http://web.uni-frankfurt.de/fb14/engels/

Esté:

August 2004. Participation in the International Drug Discovery Technology meeting, Boston, USA.

July 2004. International AIDS Congress, Bangkok, Thailand. Three poster presentations: HIV-1-resistance to ADS-J1. M. Armand-Ugón, I. Clotet, S. Jiang, B. Clotet and JA Esté. Mapping of polyanion drug -resistance to a single amino acid of the gp120 of human immunodeficiency virus type 1. I Clotet-Codina, M Armand-Ugon, B Clotet and JA Esté. Immunological and virological study of Enfuvirtide treated patients. J Barretina, A Bonjoch, J Blanco, A Llano, B Clotet and JA. Esté

April 2004. 17 th International Conference on Antiviral Research. Oral presentation: HIV resistance to binding/fusion inhibitors. J.A. Esté Poster presentation: ADS-J1, a non-peptidic low molecular weight HIV fusion inhibitor targeting gp41, with no cross-drug resistance with peptidic HIV fusion inhibitors T-20 and C-34 and HIV binding inhibitors . M. Armand-Ugón, I. Clotet, S. Jiang, B. Clotet and JA Esté

Debyser :

11 th Conference on Retroviruses and Opportunistic Infections, San Francisco, 8-11 February, 2004. Vercammen, J., De Clercq, E., Witvrouw, M., Debyser, Z. & Engelborghs, Y. Kinetics of the 3’ processing reaction of HIV-1 integrase as investigated with Fluorescence Cross Correlation Spectroscopy.

Divita :

Fifth HIV DRP Symposium on Antiviral Drug Resistance will be held November 14-17, 2004, in Chantilly, Virginia. “ Insight into the mechanism of a peptide inhibitor of HIV reverse transcriptase dimerization” Depollier, J. Hourdou, ML, Aldrian-Herrada, G. & Divita . G.

Howarth & Kovacs :

P. Pádár, M. Hornyák, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2004): Intramolekuláris 1,3-dipoláris cikloaddíció a királis azetidin nukleinsavak szintézisében. MTA Nukleotidkémiai Munkabizottsági Ülés. (An intramolecular 1,3-dipolar cycloaddition in the synthesis of chiral azetidine nucleic acids, Nucleotide Chemistry Workshop of the Hungarian Academy of Sciences, in Hungarian, May 26, Balatonszemes, Hungary) Poster.

M. Hornyák, P. Pádár, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2004): Ionos folyadékok alkalmazása 1,3-dipoláros cikloaddíciós reakciókban. MTA Nukleotidkémiai Munkabizottsági Ülés. (The application of ionic liquids in1,3-dipolar cycloaddition reactions, Nucleotide Chemistry Workshop of the Hungarian Academy of Sciences, in Hungarian, May 26, Balatonszemes, Hungary) Poster.

P. Pádár, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2004): An intramolecular 1,3-dipolar cycloaddition reaction towards the synthesis of chiral azetidine nucleoside analogues: the D-gluco case. 16th International Round Table Nucleosides, Nucleotides and Nucleic Acids, P111. (12-16 September 2004, Minneapolis, Minnesota, USA) Poster.

M. Hornyák, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2004): Investigation of the intramolecular 1,3-dipolar cycloaddition reactions of unsaturated nitrones in ionic liquids. 16th International Round Table Nucleosides, Nucleotides and Nucleic Acids, P53. (12-16 September 2004, Minneapolis, Minnesota, USA) Poster.

Kaptein:

17th EENC/32nd AMPERE meeting, Lille, September 2004 (poster).

BaCaTeC Summer School 2004, Bayreuth, October 2004 (poster).

TRIoH meeting, Messina, September 26-28, 2004 (short oral presentation)

Keppler :

European Union TRIoH consortium kick-off meeting, Lyon, France, March 6-8, 2004. Oral presentation, title: Preclinical evaluation of antiviral compounds in the human CD4/CCR5-transgenic rat model of HIV-1 infection.

Cold Spring Harbor Retrovirus Conference, Cold Spring Harbor, USA, May 25-30, 2004, poster presentation, title: Rodent cells support key functions of the HIV pathogenicity factor Nef.

Kovacs :

M. Hornyák, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2004): Investigation of the intramolecular 1,3-dipolar cycloaddition reactions of unsaturated nitrones in ionic liquids. 16th International Round Table Nucleosides, Nucleotides and Nucleic Acids, P53. (12-16 September 2004, Minneapolis, Minnesota, USA)

P. Pádár, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2004): An intramolecular 1,3-dipolar cycloaddition reaction towards the synthesis of chiral azetidine nucleoside analogues: the D-gluco case. 16th International Round Table Nucleosides, Nucleotides and Nucleic Acids, P111. (12-16 September 2004, Minneapolis, Minnesota, USA)

E. Szájli, G. Paragi and L. Kovács (2004): A study of H-bonding of 3- and 5-substituted 6-aminouracils in duplex and triplex structures. 16th International Round Table Nucleosides, Nucleotides and Nucleic Acids, P161. (12-16 September 2004, Minneapolis, Minnesota, USA)

G. Ferenc, Z. Kupihár, Z. Kele and L. Kovács (2004): A convenient method for the synthesis of oligonucleotide-cationic peptide conjugates. 16th International Round Table Nucleosides, Nucleotides and Nucleic Acids, P226. (12-16 September 2004, Minneapolis, Minnesota, USA)

G. Ferenc, Z. Kele, P. Pádár and L. Kovács (2004): MS analysis of thiazolium and pyridinium derivatives of peptides. 22nd Informal Meeting on Mass Spectrometry, MoPo21. (2-6 May, Tokaj, Hungary)

(only international events are listed)

Maga :

TRIoH meeting, Lyon ( France), March 7 – 8, 2004 (oral presentation)

Swiss HIV Workshop, Montana ( Switzerland), March 20 – 21, 2004 (oral presentation)

 Mély :

Y. MELY. NCp7 chaperone activity. TRIoH meeting. Ecole normale supérieure de Lyon, Lyon, 7-8 mars 2004.

Y. MELY. Fluorescence correlation spectroscopy with labelled nucleic acids: applications in HIV-1 biology and gene therapy. Invited seminar at the University of Chemistry, Francfort/Main, 26 mai 2004.

Y. MELY. Spectroscopie à corrélation de fluorescence. Ecole thématique interdisciplinaire « Microscopie fonctionnelle en biologie ». Ile d’Oléron, 12-17 septembre 2004.

Y. MELY. Spectroscopie à corrélation de fluorescence : examples d’applications dans le domaine de VIH-1 et de la thérapie génique. Invited conference. Troisième conférence biophotonique, Opto 2004, Paris, 20 octobre 2004.

A.S. KLYMCHENKO, G. DUPORTAIL, Y. MELY, S. ERCELEN, & AP DEMCHENKO. Multiparametric fluorescence probing of nanoscale compositions and biological systems. Oral Communication. Photonics Europe, Strasbourg, 26-30 avril 2004.

V.V. SHYNKAR, A.S. KLYMCHENKO, G. DUPORTAIL, AP DEMCHENKO, & Y. MELY. Ratiometric fluorescence measurements and imaging of the dipole potential in cell plasma membranes. Oral Communication. Photonics Europe, Strasbourg, 26-30 avril 2004.

Y. MELY, J. AZOULAY, H. BELTZ, J.P. CLAMME, S. BERNACCHI, D. FICHEUX, B.P. ROQUES & J.L. DARLIX. Investigation by two-photon fluorescence correlation spectroscopy of the interaction of the nucleocapsid protein of HIV-1 with hairpin loop DNA sequences. Oral Communication. Photonics Europe, Strasbourg, 26-30 avril 2004.

V.V. SHYNKAR, A.S. KLYMCHENKO, AP DEMCHENKO, & Y. MELY. H-bond sensing with 3-hydroxyflavones: steady-state and time-resolved fluorescence studies. Photonics Europe, Strasbourg, 26-30 avril 2004.

H. BELTZ, J. AZOULAY, J.P. CLAMME, S. BERNACCHI, D. FICHEUX, B.P. ROQUES, J.L. DARLIX & Y. MELY. Mécanisme de destabilisation de la sequence complémentaire de TAR de VIH-1 par la protéine de la nucléocapside: etude par spectroscopie de fluorescence résolue en temps et FCS. Optdiag, Paris, 11-13 mai 2004.

C. RAJA, U. DIETRICH, D. FICHEUX, B.P. ROQUES, J.L. DARLIX & Y. MELY. Inhibition of HIV-1 Nucleocapsid Protein NCp7 Chaperone Activity by Small Peptides. Journée scientifique de l’IFR Gilbert Laustriat, Illkirch, 16-17 septembre 2004 .

N. RAMALANJAONA, C. EGELE, C. RAJA, H. DE ROCQUIGNY, & Y. MELY. Chimie-physique et fluorescence : une combinaison efficace pour la modélisation de biologique.. Journée scientifique de l’IFR Gilbert Laustriat, Illkirch, 16-17 septembre 2004.

C. EGELE, E. SCHAUB, N. RAMALANJAONA, E. PIEMONT, D. FICHEUX, B. ROQUES, J. L. DARLIX, & Y. MELY. HIV-1 nucleocapsid protein binds to the viral initiation DNA sequences and chaperones their kissing interactions. Journée scientifique de l’IFR Gilbert Laustriat, Illkirch, 16-17 septembre 2004.

A.S. KLYMCHENKO, V.V. SHYNKAR, G. DUPORTAIL, AP DEMCHENKO, & Y. MELY. Solvatochromic dyes for probing organ ized systems: design and applications . Journées de Photochimie, ENS Cachan, 18-19 novembre 2004.

Y. Mély. Intérêt des techniques de fluorescence dans la caractérisation des interactions biomoléculaires: applications dans le domaine de VIH et de la thérapie génique. Faculté des Sciences et Techniques, Université de Franche-Comté, Besançon, 13 janvier 2005.

Y. Mély. Structure-activity relationships of the nucleic acid destabilizing properties of NCp7. TRIoHgeneral assembly. Georg-Speyer Haus, Frankfurt/Main, March 21-22, 2005.

Y. Mély. Nucleocapsid protein: a potential target for therapy. TRIoH Scientific Symposium. Campus of the University Clinics, Frankfurt/Main, March 23th, 2005.

Y. Mély. Analysis by FCS of the chaperone properties of the nucleocapsid protein of HIV-1. European worksop on fluorescence correlation spectroscopy techniques. Faculty of Medecine, Le Kremlin-Bicêtre, Paris, March 23-24th, 2005.

C. EGELE, E. SCHAUB, N. RAMALANJAONA, E. PIEMONT, D. FICHEUX, B. ROQUES, J.L. DARLIX, & Y. MELY. HIV-1 nucleocapsid protein binds to the viral initiation DNA sequences and chaperones their kissing interactions. TRIoH general assembly. Georg-Speyer Haus, Frankfurt/Main, March 21-22, 2005.

A. HAC, J. GODET, H. BELTZ, & Y. MELY. Promotion of cTAR DNA homodimers by the nucleocapsid protein of HIV-1 virus. European worksop on fluorescence correlation spectroscopy techniques. Faculty of Medecine, Le Kremlin-Bicêtre, Paris, March 23-24th, 2005.

Restle:

13th International Symposium on HIV & Emerging Infectious Diseases, June 3-5, 2004, Toulon, France

Symposium "THE RETROVIRUS-HOST INTERFACE: FROM STRUCTURE TO FUNCTION" 30. Sept - 2.Okt 2004, Bayreuth, Germany - poster

Congress on "Peptide-Membrane Interactions" October 9-12, 2004 in Namur, Belgium - poster

Symposium "Peptides in Drug Delivery" November 25 - 27, Montpellier, France - oral presentation

Robina :

Dr. Antonio Moreno Vargas and Mr. M. Bautista (PhD student) went the 18 th, February, 2004 for one week to the 6 th Combinatorial Chemistry week in Mollet del Valles in BCN where they got a deeper insight about the Principles and experimental of Combinatorial Chemistry, parallel and solid-phase synthesis. This training course had been organised by the Instituto Universitario de Cienia y Tecnología of Spain. We found this training course very convenient for our synthetic contribution to the project.

Vilarrasa :

Ischia Advanced School of Organic Chemistry, 18-23 Sept 2004, Poster of M Terrazas (on her syntheses of modified nucleosides).

Workshop of the Medicinal Chemistry Network, 17 Nov. 04, Univ. Barcelona (participation of several members of the group).

Villalain :

XXVII Congress of the SEBBM, Lérida, Spain, 2004. Two posters.

48 th Biophysical Society Meeting, 2004, Baltimore, USA. Oral presentation.

Witvrouw:

11 th Conference on Retroviruses and Opportunistic Infections, San Francisco, 8-11 February, 2004. Poster presentation: Witvrouw, M., Fikkert, V., Van Remoortel, B., Van Maele, B., De Clercq, E. &. Debyser, Z. Multiple Mutations in Human Immunodeficiency Virus- 1 Integrase Confer Resistance to the Phase I/II Clinical Trial Drug S-1360

17th ICAR, Tucson, Arizona, USA, 2-6 May, 2004. Oral presentation: Witvrouw, M., Fikkert, V., Pannecouque, C., Van Remoortel, B., E. De Clercq, E., & Debyser, Z. Multiple Mutations in Human Immunodeficiency Virus-1 Integrase Confer Resistance to the Phase I/II Clinical Trial Drug S-1360.

5th HIV DRP Symposium – Antiviral Drug Resistance, Chantilly, Virginia, USA, 14-17 November, 2004. Oral presentation: Witvrouw, M., Hantson, A., Hombrouck, A., Busschots, K., Tetz, V., Pannecouque, C., De Clercq, E., Van Maele, B. & Debyser, Z. Multiple Mutations in Human Immunodeficiency Virus-1 Integrase Confer Resistance to the Pyranodipyrimidine V-165.

2005

Witvrouw & Debyser:

F.Christ, L. Vandekerckhove, B. Van Maele, S. Emiliani, R. Benarous , M. Witvrouw and Z. Debyser. RNA Interference as a Validation Tool for LEDGF/p75 and Potential Other Co-factors for HIV-1 Replication. TrioH-meeting Frankfürt (2005).

Van Maele, B., Vandekerckhove, L., Busschots, K., De Rijck, J., Emiliani, S.*, Benarous R, Witvrouw M, Gijsbers R, Christ F, Debyser Z. A role for the cellular co-factor, LEDGF/p75 in targeting HIV-1 integration?. TrioH-meeting Frankfürt (2005)

Hantson A. Hombrouck A., Voet A. , Vercammen J., Engelborghs Y. , De Mayer M., Witvrouw M. and Debyser Z. Importance of amino acids V165, T206, and N155 for the interaction of pyranodipyrimidines with HIV-1 Integrase as revealed by molecular docking and resistance selection, TrioH-meeting Frankfürt (2005).

Hantson, A., Hombrouck, A., Vercammen, J., Tetz, V., Pannecouque, C., Engelborghs, Y., De Clercq, E., Debyser, Z. & Witvrouw, M., The V165I and T206S/S230N Mutations in Human Immunodeficiency Virus-1 Integrase Confer Resistance to the Pyranodipyrimidine V-165 and Reduce Replication Capacity.12th Conference on Retroviruses and Opportunistic Infections, Boston, USA, 22-25 February, 2005.

L. Vandekerckhove, F. Christ, K Busschots, Jo Vercammen, S. Emiliani, R. Benarous, M. Witvrouw, Y. Engelborghs and Z. Debyser LEDGF/p75 is a Co-Factor of Lentiviral Integrases and Plays a Role in HIV Replication 12th Conference on Retroviruses and Opportunistic Infections, Boston, USA, 22-25 February, 2005.

F.Christ, L. Vandekerckhove, S. Emiliani, R. Benarous, M. Witvrouw and Z. Debyser. Validation of HIV-1 Integrase Co-factor using RNA Interference. 12th Conference on Retroviruses and Opportunistic Infections, Boston, USA, 22-25 February, 2005.

M. Witvrouw, A. Hantson, A. Hombrouck, K. Busschots, V. Tetz, C. Pannecouque, E. De Clercq, and Z. Debyser. The V165I and T206S/S230N Mutations in Human Immunodeficiency Virus-1 Integrase Confer Resistance to the Pyranodipyrimidine V-165 and Reduce Replication Capacity. The 12th Conference on Retroviruses and Opportunistic Infections. February 22-25, 2005 , Boston, US.

Arnout R.D. Voet, Bénédicte Van Maele, Christophe Pannecouque, Erik De Clercq, Myriam Witvrouw, Zeger Debyser and Marc De Maeyer. Active site binding modes of Pyranodipyrimidines, a class of authentic HIV-1 integrase inhibitors. 4th Johnson & Johnson Pharmaceutical Research & Development Symposium on Drug Discovery, held at the University of Antwerp (UA), Belgium, 7-8 April, 2005.

Hombrouck, A. Hantson, J. Vercammen, V. Tetz, C. Pannecouque, E. De Clercq, B. Van Maele, Y. Engelborghs, Z. Debyser and M. Witvrouw Multiple Mutations in Human Immunodeficiency Virus-1 Integrase Confer Resistance to the Pyranodipyrimidine V-165 Cold Spring Harbor Laboratory Meeting-USA-Retroviruses, 24-29 May, 2005.

L. Vandekerckhove, J. De Rijck, K. Busschots, J. Vercammen, B. Van Maele, S. Emiliani, R. Benarous, Y. Engelborghs, R. Gijsbers, M. Witvrouw, F. Christ and Z. Debyser. Validation of LEDGF/p75 as an important co-factor of lentiviral integration. INVESTIGATOR'S AWARD "Belgian Society of Internal Medicine " 9-10 decembre, 2005.

Vandekerckhove L, Busschots K, Vercammen J, Van Maele B, De Rijck J, Voet A, Emiliani S, Benarous R, De Maeyer M, Engelborghs Y, Gijsbers R, Witvrouw M, Christ F, Debyser Z. Validation of LEDGF/p75 as an important co-factor of lentiviral integration. 45th ICAAC, Washington D.C., 16-19 December 2005. Cold Spring Harbor Laboratory Meeting-USA-Retroviruses, 24-29 May, 2005.

Esté:

Inhibition of Co-receptor-independent Cell-to-cell HIV-1 Transmission. Berta Bosch, J Blanco, M Fernandez-Figueras, B Clotet, and J Este (2005) 12th Conference on Retroviruses and Opportunistic Infections (CROI), Boston ( USA). Cold Spring Harbour Laboratory Meeting on Retroviruses, NY (USA).

Alpha-v integrins intervene in human immunodeficiency virus type 1 (HIV-1) infection in macrophages. Berta Bosch, Julià Blanco, Samandhy Cedeño, Francesc Mitjans, Anuska Llano, Bonaventura Clotet, Jaume Piulats, José A. Esté. 6th Int Workshop on HIV Cells of Macrophage/Dendritic Lineage & Other Reservoirs, Varenna, Italy  5-7 October 2005.

A Multiparametric assay to screen and dissect the mode of action of anti-HIV drugs. J Blanco, I Clotet-Codina, B Bosch, M Armand-Ugón, B Clotet, and JA Esté. Int. Conference on Antiviral Research, Barcelona April 2005.

Virological synapse formation preferentially targets CD45RO+ CD4 T cells and determines the mechanism of HIV internalization. J Blanco, B Bosch, R Ruiz, M Bofill, B Clotet and J A. Esté. 1ST Meeting on HIV entry. Bethesda, USA.

Darlix:

9/02/2005. General conference on HIV NC and viral RNA chaperones. Mérieux INC. Lyon, France.

14/02/2005. Course on HIV assembly within the framework of an European course on Virus & Immunity.

29/03/2005. Conference on ‘HIV and the people’ for a large audience at the Ecole Centrale, Lyon

6/04/2005. Conference on ‘HIV and the people’ for a large audience at INSA, Lyon

31/05/2005. Conference on HIV assembly and the role of NC at IGBMC, Strasbourg.

13/06/2005. Conference on HIV NC and other viral chaperones, at the University of Vienna, Austria.

14/09/2005. Conference on HIV assembly at the NIH, Bethesda, USA.

15/09/2005. Conference on the functions of NC at the NCI, Frederick.

16/09/2005. Conference on HIV NC and HCV Core, at the University of Baltimore.

10/10/2005. HIV and the people, a general conference for medical doctors involved in the treatment of drug addiction; Arles TSH7.

14/10/2005. General conference on HIV at the annual meeting of the Chilean Society of Biology, Pucon, Chile.

28/10/2005. Course on HIV at the Faculty of Medicine of Santiago de Chile.

14/11/2005. Conference on HIV NC and the screening of anti-NC, at the Commissariat à l’Energie Atomique, Saclay, France.

12/2005-07/2006. Exhibition on ‘Virus & Camouflages’, Lyon Museum.

Club Exocytose-Endocytose, Giens, France, June 2005. B.Grigorov, F.Arcanger, P.Roingeard, J.-L. Darlix and D.Muriaux “Intracellular formation of infectious HIV-1 particles involved localization of Gag, Env and the genomic RNA in late endosomes”.

The 2005 Meeting on Retroviruses. Cold Spring Harbor Laboratory, CSH, New York, May 2005. OK

Grigorov, B., Arcanger, F., Roingeard, P., Darlix, J.-L. and Muriaux, D. « Intracellular budding of infectious HIV-1 particles».

Loraine Jarrosson-Wuilleme, Caroline Goujon, Jeanine Bernaud, Dominique Rigal, Jean-Luc Darlix and Andrea Cimarelli. Challenging the impermeability of the nuclear membrane to oncoretroviral infection: efficient transduction of human macrophages with a Friend murine leukemia derived vector.

Caroline Goujon, Loraine Jarrosson-Wuilleme, Jeanine Bernaud, Dominique Rigal, Jean-Luc Darlix and Andrea Cimarelli. SIVMAC VPX counteracts an anti-lentiviral activity specifically present in human dendritic cells.

Carole Bampi1*, Stuart Le Grice2, Gilles Divita3, Juan José Martínez-Irujo4, Jean-Luc Darlix. new insights into the roles of reverse transcriptase and nucleocapsid protein in the genetic variability of hiv-1

Loraine Jarrosson-Wuilleme, Caroline Goujon, Jeanine Bernaud, Dominique Rigal, Jean-Luc Darlix and Andrea Cimarelli. MEETING ON GENE THERAPY ( LYON, FRANCE) An old dogma revisited: gammaretroviruses efficiently transduce non-dividing primary human macrophages.

18-20/09/2005. Co-organizer of the Fifth International retroviral NC symposium, Montréal, Canada, September 2005.

D.Muriaux, D.Décimo, B.Grigorov, C.Péchoux and J.-L. Darlix. “Role of nucleocapsid zinc fingers in HIV-1 Gag assembly and trafficking”.

Carole Bampi, Stuart Le Grice, Gilles Divita, Juan José Martínez-Irujo, Jean-Luc Darlix. New insights into the roles of reverse transcriptase and nucleocapsid protein in the genetic variability of hiv-1.

Boyan Grigorov1, Fabienne Arcanger2, Philippe Roingeard2, Jean-Luc Darlix1 and Delphine Muriaux Intracellular formation of infectious HIV-1 particles

Delphine Muriaux, Didier Décimo, Boyan Grigorov, Christine Péchoux and Jean-Luc Darlix. Role of nucleocapsid zinc fingers in HIV-1 Gag assembly and trafficking

Leblanc P, Alais S, Porto-Carreiro I, Lehmann S, Grassi J, Raposo G & JL Darlix. Retroviruses enhance prion proteins release Dominique DORMONT CONFERENCE (PARIS, FRANCE)

Debyser

Arnout R.D. Voet, Abel Jonckheer, Katrien Busschots, Frauke Christ, Zeger Debyser and Marc De Maeyer. MODELING OF THE INTEGRASE BINDING DOMAIN OF LEDGF/P75 AND IN COMPLEX WITH HIV-1 INTEGRASE. TrioH-meeting Frankfürt (2005).

De Rijck J. and Debyser Z. The HIV-1 DNA flap stimulates both the efficiency and the kinetics of lentiviral vector transduction. Cold Spring (2005.)

Benarous & Debyser :

Benarous, R. , Van Maele, B., Mousnier, A ., Maroun, M ., Vandekerckhove, L ., Busschots, K ., Witvrouw, M . , Rain, J-C . , Dargemont, C ., Debyser, Z . & Emiliani, S . LEDGF/p75 is involved in targeting of HIV-1 integrase to chromosomes in integration and viral replication.12th Conference on Retroviruses and Opportunistic Infections, Boston, USA, 22-25 February, 2005.

K. Busschots, J. Vercammena, S. Emiliani, Y. Engelborghs, F. Christ, R. Benarous and Z. Debyser. THE INTERACTION OF LEDGF/p75 WITH INTEGRASE IS LENTIVIRAL-SPECIFIC AND PROMOTES DNA BINDING. TrioH-meeting Frankfürt (2005) Poster presentation.

Chimirri

Alba Chimirri, Maria Letizia Barreca, Laura De Luca, Stefania Ferro, Anna-Maria Monforte,Angela Rao. Computation al and synthetic approaches for the discovery of HIV-1 integrase inhibitors:. 20 th International Congress of Heterocyclic Chemistry. July 31 Luglio – August 5, Palermo ( Italy) 2005, pp1-IL10-10

Laura De Luca, Maria Letizia Barreca, Stefania Ferro, Angela Rao, Anna-Maria Monforte and Alba Chimirri , “ Pharmacophore-Based Design of HIV-1 Integrase Strand-Transfer Inhibitors”, AccelrysWorld, London (UK), Nov 14 - 16, 2005

Chimirri, Witvrouw & Debyser:

Maria Letizia Barreca, Stefania Ferro, Angela Rao, Laura De Luca, Maria Zappalà,, Anna-Maria Monforte, Zeger Debyser, Myriam Witvrouw, Alba Chimirri. HIV-1 integrase inhibitors: pharmacophore modelingand rational drug design. Joint Meeting on Medicinal Chemistry, Vienna ( Austria), June 20-23 2005, PO-126.

Stefania Ferro, Maria Letizia Barreca, Angela Rao, Laura De Luca, Anna-Maria Monforte, Zeger Debyser, Myriam Witvrouw, Alba Chimirri. Design e sintesi di nuovi inibitori dell’integrasi dell’HIV-1. XII Convegno Nazionale sulle reazioni pe ricicliche e sintesi di etero e carbocicli. Messina (Italy) September 8-9, pp39-40.

Stefania Ferro, Maria Letizia Barreca, Angela Rao, Laura De Luca, Anna-Maria Monforte, Zeger Debyser, Myriam Witvrouw , and Alba Chimirri; “Drug design and synthetic approach for the discovery of novel HIV- integrase inhibitors” Second Joint meeting on Medicinal Chemistry, Modena (Italy), September 12-16 2005, pp121

S. Ferro, M. L. Barreca, L. De Luca, A. Rao, A.M. Monforte, P. Monforte, Z. Debyser, M. Witvrouw and A. Chimirri “Progettazione razionale e “green” sintesi di nuovi inibitori dell’integrasi dell’HIV-1” Convegno congiunto delle Sezioni Calabria e Sicilia 2005 – Società Chimica Italiana, Catania (Italy) December 5-6 2005

Dietrich :

27.2.-2.3.2005: 7 th German Peptide Symposium, Braunschweig, Germany. Oral presentation by U. Dietrich: “Stabilization of therapeutic peptides by multimerization”

29.8.-2.9.2005: 10th International Meeting of the Institute of Human Virology, Baltimore, MD, USA. Poster presentation by Humbert M, Antoni S, Schreiber A, Rodes B, Soriano V, Dittmar M, Dietrich U. Phage display selection of HIV specific conserved mimotopes with IgG from long-term non-progressors OK

6.-8.10.2005: Volkswagen Meeting “Conformational control of Biomolecular Function”, Heidelberg, Germany. Oral presentation by U.Dietrich. OK

Dervillez X, Königs C, Hüther A, Pustowka A, Loderstädt U, Klaukien V, Griesinger C, Dietrich U. Stabilization of therapeutic peptides by multimerization.

Dervillez X, Klaukien V, Königs C, Wegner V, Griesinger C, Dietrich U. Characterization of HIV-1 entry domains and their peptide ligands

Engels :

J.W.Engels, RSC, Nucleic Acid Chemistry Towards Biology: 50 Years On, Cambridge 9 th December 2005

Satellite Symposium on Nucleic Acids Chemistry, 8th Dec. 2005, MRC Laboratory of Molecular Biology, Cambridge, U.K (oral presentation): RNA modifications and analogues to address biological questions.

Gottikh :

TRIoH Scientific Symposium, 20-24 March 2005, Frankfurt, Germany. Maksim Smolov, Timofei Zatsepin, Julie Agapkina, Malcolm Buckle, Jean-François Mouscadet and Marina Gottikh. Characterization of DNA binding by HIV-1 integrase using Schiff-base formation. Poster.

TRIoH Scientific Symposium, 20-24 March 2005, Frankfurt, Germany. Prikazchikova T., Volkov E., Romanova E., Mouscadet J.-F. and M. Gottikh. Inhibition HIV-1 Integrase catalytic activity by oligonucleotide conjugates. Poster.

30th FEBS Congress and 9th IUBMB Conference “The Protein World”, 2-7 July 2005, Budapest, Hungary. J. Agapkina, M. Smolov and M. Gottikh. Interactions of HIV-1 integrase with modified analogs of viral DNA: implications for understanding integration mechanism. Poster.

30th FEBS Congress and 9th IUBMB Conference “The Protein World”, 2-7 July 2005, Budapest, Hungary. T. Prikazchikova, J.-F. Mouscadet and M. Gottikh. Integrase HIV-1: inhibition of catalytic activity by modified oligonucleotides. Poster.

30th FEBS Congress and 9th IUBMB Conference “The Protein World”, 2-7 July 2005, Budapest, Hungary. M. Smolov, J. Agapkina, M. Buckle, J.-F. Mouscadet and M. Gottikh. Characterization of DNA binding by HIV-1 integrase using Schiff-base formation. Poster.

International Symposium “Advances in Science for Drug Discovery”, 11-16 July 2005, Moscow, Russia. Tatiana Prikazchikova, Julie Agapkina, Marina Gottikh. Integrase HIV-1: inhibition of catalytic activity in vitro. Poster.

International Symposium “Advances in Science for Drug Discovery”, 11-16 July 2005, Moscow, Russia. Maksim Smolov, Eric Deprez and Marina Gottikh. HIV-1 integrase acts as a single turnover enzyme. Poster.

Howarth:

TRIoH 2nd General Assembly, 21st-22nd March 2005, Frankfurt, Germany (2 poster presentations).

K.M. Collart and N.M. Howarth. Approaches Towards Potential Novel Integrase Inhibitors: PNA-Acridine Threading Intercalators.

K.F. Adebambo and N.M. Howarth. PNA Oligomers as Potential Reverse Transcriptase or Integrase Inhibitors: Synthesis of Guanine PNA monomer.

3rd University of Glasgow-WestChem/Organon Symposium on Synthetic Chemistry, 1st Sept. 2005, University of Glasgow, Scotland (1 poster presentation).

K.F. Adebambo and N.M. Howarth. Guanine-rich PNA Oligomers as Potential HIV-1 Integrase Inhibitors: Synthesis of Guanine PNA monomers. Poster.

RSC Bioorganic and Nucleic Acid groups Autumn Meeting, 9th-11th Sept. 2005, Firbush, Loch Tay, Scotland (oral presentation).

Satellite Symposium on Nucleic Acids Chemistry, 8th Dec. 2005, MRC Laboratory of Molecular Biology, Cambridge, U.K (oral presentation): Alpha-amino acid based peptide nucleic acids .

RSC Perkin Division: 34th Annual Scottish Regional Meeting, 19th Dec. 2005, University of Strathclyde, Scotland.

N. Howarth. PNA-functionalised PDAs: Potential Nucleic Acid Biosensors. Oral Presentation.

K.F. Adebambo and N.M. Howarth. Synthesis and Biological Evaluation of Novel Guanine Derivatives as Potential HIV-1 Integrase and Reverse Transcriptase Inhibitors. Poster.

K.M.P. Collart and N.M. Howarth. Synthetic Approaches Towards Potential Novel HIV-1 Integrase Inhibitors: PNA-Acridine Threading Intercalators. Poster.

 

Keppler

Annual meeting of German society for virology, Hannover, Germany, March 16-19, 2005. Poster title: Analysis of Restrictions in the HIV Replication Cycle in Primary Rat T-Cells: Advancing the Transgenic Rat Model of HIV Infection by Christine Goffinet, Barbara Müller, Hans-Georg Kräusslich and Oliver T. Keppler.

2nd international workshop on HIV persistence during therapy, St. Martin, French West Indies, December 6-9, 2005. Title of oral presentation: The role of macrophages in HIV persistence.

Kovacs :

L. Kovács (2005): A minimalist introduction to (bio)organic chemistry. Targeting Replication and Integration of HIV Scientific Symposium. (March 23, Campus of the University Clinics, Frankfurt am Main, Germany, lecture)

M. Hornyák, P. Forgó, N. M. Howarth, Z. Kele and L. Kovács (2005): 1,3-Dipoláros cikloaddíciós reakciók sztereoszelektivitásának vizsgálata ionos folyadékban. MTA Nukleotidkémiai Munkabizottsági Ülés. (The study of stereoselectivity of 1,3-dipolar cycloadditions in ionic liquids, Annual Nucleotide Chemistry Workshop of the Hungarian Academy of Sciences, in Hungarian, May 30-June 1, Balatonszemes, Hungary, lecture)

Z. Kele, É. Klement, G. Ferenc, P. Pádár, G. K. Tóth and L. Kovács (2005): Brómot és kvarterner nitrogént tartalmazó reagensek. MTA Peptidkémiai Munkabizottsági Ülés. (Reagents containing bromine and quaternary nitrogen, Annual Peptide Chemistry Workshop of the Hungarian Academy of Sciences, in Hungarian, May 30-June 1, Balatonszemes, Hungary, lecture)

P. Pádár, A. Bokros, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2005): Újabb eredmények D-glükózból származó telítetlen nitronok intramolekuláris 1,3-dipoláris cikloaddíciós reakcióiban. MTA Nukleotidkémiai Munkabizottsági Ülés. (Recent results in the intramolecular 1,3-dipolar cycloaddition reactions of nitrones derived from D-glucose, Annual Nucleotide Chemistry Workshop of the Hungarian Academy of Sciences, in Hungarian, May 30-June 1, Balatonszemes, Hungary, lecture)

P. Pádár, M. Hornyák, A. Bokros, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2005): Intramolekuláris 1,3-dipoláris cikloaddíciós reakciók szénhidrátokból származó telítetlen nitronok körében. MKE Vegyészkonferencia , O-3. (Annual Conference of the Hungarian Chemical Society, in Hungarian, June 28-30, Hajdúszoboszló, Hungary, lecture)

P. Pádár, M. Hornyák, A. Bokros, P. Forgó, Z. Kele, N. M. Howarth and L. Kovács (2005): Intramolecular 1,3-dipolar cycloadditions of carbohydrate-derived unsaturated nitrones. 2nd Austrian-Hungarian Carbohydrate Conference (May 24-26, Somogyaszaló, Hungary, lecture)

Z. Kele, G. Ferenc, É. Klement, P. Pádár, G. K. Tóth and L. Kovács (2005): CE-MS/MS analysis of bromo-containing derivatives of peptides. 23nd Informal Meeting on Mass Spectrometry, May 15-19, Fiera di Primiero, Italy, poster.

Massa :

Mugnaini C, Radi M, Corelli F, Botta M. Synthesis of reactive cytidine derivatives as building blocks for cross-linking oligonucleotides. (Poster) FEBS Summer-School Chemistry meets biology. Spetses Island (Greece) July, 17-29 2005

Elena Petricci, Marco Radi, Federico Corelli and Maurizio Botta. Synthesis of new non nucleoside compounds as potential HIV-1 RT inhibitors. (Poster) FEBS Summer-School Chemistry meets biology. Spetses Island ( Greece) July, 17-29 2005

Ciccioni, C.; Mugnaini, C.; Petricci, E.; Botta, M.; Corelli, F. Sintesi assistita da microonde di una piccola libreria di 2-ariltio-pirimidinoni tramite reazione di condensazione di Ullmann modificata” (Poster) XXX Convegno nazionale della divisione di chimica organica. Siena, 19-23 September

Mély :

Y. MELY: Intérêt des techniques de fluorescence dans la caractérisation des interactions biomoléculaires : applications dans le domaine de VIH et de la thérapie génique. Faculté des Sciences et Techniques, Université de Franche-Comté, Besançon, 13 janvier 2005.

Y. MELY: Analysis by FCS of the chaperone properties of the nucleocapsid protein of HIV-1. European worksop on fluorescence correlation spectroscopy techniques. Faculty of Medecine, Le Kremlin-Bicêtre, Paris, March 23-24th, 2005.

Y. MELY : Rôle de NCp7 dans les sauts de brin: mécanisme, relation structure-activité et criblage. AC14 “ Nouvelles cibles thérapeutiques ” Thème “ Protéines régulatrices et Assemblage, bourgeonnement du VIH ”. ANRS, 27 juin 2005.

Y. MELY: Structural determinants and mechanistic aspects of NCp7 chaperone activity in reverse transcription. 5th International Retroviral NC Symposium. Montreal, September 18-20, 2005.

Y. MELY: Etude biophysique du rôle de la protéine de la nucléocapside du virus du SIDA dans la transcription inverse, IPCMS, Strasbourg, 3 novembre, 2005.

A. HAC, J. GODET, H. BELTZ, & Y. MELY. Promotion of cTAR DNA homodimers by the nucleocapsid protein of HIV-1 virus. European worksop on fluorescence correlation spectroscopy techniques. Faculty of Medecine, Le Kremlin-Bicêtre, Paris, March 23-24th, 2005.

M.D. BILOKIN, D.A.YUSCHENKO, O.V. PIVOVARENKO, G. DUPORTAIL, Y. MELY, & V.G. PIVOVARENKO. Synthetic pathways to 2’-[4-(dialkylamino)phenyl]-3-hydroxyquinolones. The 3rd joint conference in Chemistry. Kiev , May 18-22, 2005.

D.A.YUSCHENKO, M.D. BILOKIN, O.V. PIVOVARENKO, G. DUPORTAIL, Y. MELY, & V.G. PIVOVARENKO. Synthesis and fluorescence properties of 2-aryl-3-hydroxyquinolones. The 3rd joint conference in Chemistry. Kiev , May 18-22, 2005.

A. HAC, J. GODET, H. BELTZ, & Y. MELY. Promotion of cTAR DNA homodimers by the nucleocapsid protein of HIV-1 virus. Vth EBSA International Biophysics Congress, Montpellier 27 Août-1er Septembre 2005.

V.V. SHYNKAR, A.S. KLYMCHENKO, A.P. DEMCHENKO, Y. MELY & G. DUPORTAIL. " Two-color fluorescent probes for imaging dipole potential of cell plasma membranes." IXth International Conference on Methods and Application of Fluorescence (MAF9), Lisbonne, September 4-7, 2005.

S. ONCUL, K. BAYSAL, A.S. KLYMCHENKO, Y. MELY, G. DUPORTAIL & A.P. DEMCHENKO. " Hydrophobic 3-Hydroxyflavone derivatives as biomembrane probes. "IXth International Conference on Methods and Application of Fluorescence (MAF9), Lisbonne, September 4-7, 2005.

H. BELTZ, C. EGELE, N. RAMALANJAONA, E. PIEMONT, E. SCHAUB, D. FICHEUX, J.L. DARLIX, H.de ROCQUIGNY, & Y. MELY. Investigation by FCS and time-resolved fluorescence spectroscopy of the chaperone properties of the nucleocapsid protein of HIV-1. IXth International Conference on Methods and Application of Fluorescence (MAF9), Lisbonne, September 4-7, 2005.

C. EGELE, N. RAMALANJAONA, B.P. ROQUES, J.L. DARLIX, H.de ROCQUIGNY, & Y. MELY. NCp7 binding and annealing activities during the second strand transfer. 5th International Retroviral NC Symposium. Montreal, September 18-20, 2005.

Mouscadet:

JF Mouscadet. Courses on viruses and antivirals given to Chinese students at ECNU (Shangai) 7-20 March 2005

J. C. Brochon. French-Vietnam school in photonics and application - Hanoi 6-9 December 2005.“Application of time-resolved fluorescence anisotropy in the study HIV-1 integrase” in vitro and its inhibitor molecules. http://www.iop.vast.ac.vn/activities/vnfr/detail.htm

JF Mouscadet. Resistance and synergies meeting October 13-14, 2005. New development on integrase inhibitors – invited speaker.

Guiot E., Delelis O., Deprez E., Simon F., Mbemba G., Mouscadet J-F. & Brochon J-C. Study of the 3’-processing reaction catalyzed by HIV-1 integrase using steady-state fluorescence anisotropy. TRIoH symposium, Frankfurt, Germany, 21-23 march 2005.

Delelis O., Guiot E., Deprez E., Tauc P., Brochon J-C. & Mouscadet J-F. Specific fluorescent labelling of HIV-1 integrase for probing protein-protein interactions by fluorescence correlation spectroscopy. TRIoH symposium, Frankfurt, Germany, 21-23 march 2005.

Deprez E., Guiot E., Tauc P., Simon F., Delelis O., Mouscadet J-F. & Brochon J-C. Steady-state and time-resolved anisotropy studies of HIV-1 integrase: insight into the mechanism of the 3’-processing reaction. 15th IUPAB & 5th EBSA International Biophysics Congress, Montpellier, France, 27 august-1 september 2005.

Deprez E., Guiot E., Tauc P., Simon F., Delelis O., Mouscadet J-F. & Brochon J-C. Steady-state and time-resolved anisotropy studies of HIV-1 integrase: insight into the mechanism of the 3’-processing reaction. 9th International Conference on Methods and Applications of Fluorescence, Lisboa, Portugal, 4-7 september 2005.

Guiot E., Deprez E., Tauc P., Delelis O., Leh H., Mouscadet J-F. & Brochon J-C. Mechanistic study of HIV-1 integrase by fluorescence correlation spectroscopy and time-resolved fluorescence anisotropy. 9th International Conference on Methods and Applications of Fluorescence, Lisboa, Portugal, 4-7 september 2005.

Restle :

International Symposium on ”Cell-penetrating peptides and applications”, Stockholm - Sweden, May 18-21, 2005.

Satellite meeting of the joint 15th IUPAB & 5th EBSA International Biophysics Congress, „Mechanisms of Carrier-Mediated Intracellular Delivery of Therapeutics“, Montpellier - France, August 25 & 26, 2005 – poster

International Murnau Conference on Structural Biology and Molecular Recognition, Murnau - Germany, September 15–17, 2005 – poster

International GBM Annual Fall Meeting Berlin - Germany, September 18-21 , 2005 – poster.

Robina :

A. J. Moreno-Vargas, A. T. Carmona, F. Mora,P. Vogel and I. Robina. Different approaches for stereoselective syntheses of 2-benzymidazolyl-3,4-dihydroxy-5-methylpyrrolidines. Evaluation of the inhibitory activity towards glycosidases Workshop CostD25: Applied biocatalyst, Barcelona ( Spain ),5-7 April, 2005.

A. T. Carmona, P. Fialova, V. Kren, A. J. Moreno-Vargas and I. Robina. Cyanodeoxy-Glycosyl derivatives as substrates for enzymatic transglycosylations. Workshop CostD25: Applied biocatalyst, Barcelona ( Spain), 5-7 April, 2005.

A. J. Moreno-Vargas, A. T. Carmona, F. Mora,P. Vogel and I. Robina. Different approaches for stereoselective syntheses of 2-benzymidazolyl-3,4-dihydroxy5-methylpyrrolidines. Evaluation of the inhibitory activity toward glycosidases. Glupor6, III Iberian Carbohydrate Meeting, Coimbra Portugal , Sep 2005. PC-39, pp. 87

A. T. Carmona, A. J. Moreno-Vargas, L. Molina, B. Picot, J. L. Espartero, I. Robina. Synthesis of w -aminoacids bearing polyhydroxyalkylfurans. An approach to trivalent glycoconjugate clusters. Glupor6, III Iberian Carbohydrate Meeting, Coimbra Portugal , Sep 2005, PC-38, pp. 86.

I. Robina, A. J. Moreno, A. T. CarmonaGlycomimetics as molecular tools for biology and medicine: search for new enzymatic inhibitors and anti-HIV agents. Glupor6, III Iberian Carbohydrate Meeting, Coimbra Portugal , Sep 2005 Invited Lecture, IL-38 pp. 19

J. Moreno-Vargas, A. T. Carmona, L. Molina, A. Ferrali, B. Picot, I. Robina. Synthesis of hetarylene-peptidomimetics. An approach to heterocyclicaminopolyol scaffolds for RNA binding. 4th Swiss Cost Chemistry Symposium, Lausanne ( Switzerland), October, 12, 2005, Chimia, 2005, 59, 690

A. T. Carmona,A. J. Moreno-Vargas,A. Szczepanska, J. L. Espartero, I. Robina. Synthesis of trivalent glycoconjugate clusters as CD4 binding pocket mimetics, 4th Swiss Cost Chemistry Symposium, Lausanne ( Switzerland), October, 12, 2005, Chimia, 2005, 59, 689.

T. Carmona, A. J. Moreno-Vargas, F. Mora, P. Vogel and I. Robina. Different approaches for styereoselctive Syntheses of 2-Benzymidazolyl-3,4-dihydroxy-5-methylpyrrolidines. Evaluation of the Inhibitory Activity towards Glycosidases. 4th Swiss Cost Chemistry Symposium, Lausanne ( Switzerland), October, 12, 2005, Chimia, 2005, 59, 688.

Villalain :

Interaction of a peptide comprising the C-ter region of the NHR region and the loop of HIV gp41 with model membranes", 49th Biophysical Society Meeting, Los Angeles, 2005. M. Contreras, R. Pascual, J. Villalaín

2006

Esté

• Editors of AIDS awarded the " AIDS most highly cited author award 2006" for the Basic Science section of the journal to José Esté at XVI International AIDS Conference (AIDS 2006), August 13-19, Toronto.
• B Bosch, R Peña, M Bofill, B Clotet, JA Esté. Cell to Cell HIV-1 Transfer by Dendritic Cells Depends on Virus Replication in DCs and is Blocked by Inhibitors of HIV Replication. International Workshop in HIV Drug-resistance and treatment strategies. Sitges, Spain 2006. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Este J. Entrada viral en la patogenesis del VIH. XI Jornadas Monográficas de la Sociedad Española de Inmunología. 24-25 de Noviembre de 2006. San Lorenzo de El Escorial, Madrid
• Gemma Moncunill, Mercedes Armand-Ugón, Imma Clotet-Codina, Bonaventura Clotet, Anuska Llano, José A. Esté. Development of potent anti-HIV inhibitors targeting CXCR4. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Gemma Moncunill, Mercedes Armand-Ugón, Eduardo Pauls, Bonaventura Clotet, Anuska Llano, José A. Esté. HIV-1 coreceptor switch induced by antagonism to CCR5. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Imma Clotet-Codina, Berta Bosch, Ruth Peña, Bonaventura Clotet, Margarita Bofill, José A. Esté. DC-SIGN is not required for HIV-1 transmission to CD4+ T lymphocytes. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Samandhy Cedeño, Berta Bosch, Gemma Moncunill, Jordi Senserrich, Eduardo Pauls, Anuska Llano, Francesc Mitjans, Mercedes Armand-Ugón, Imma Clotet-Codina, Bonaven­tura Clotet, Jaume Piulats, José A. Esté. Anti-HIV activity of integrin ligands. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Esté and Darlix

• Bosch B, Grigorov B, Senserrich J, Blanco J, Clote t B, Darlix J-L, Muriaux D, Este J. Cell-to-cell HIV-1 transmission through a clathrin-dependent endocytic pathway. XVI International AIDS Conference (AIDS 2006), August 13-19, Toronto

Darlix:

• February 2006: Master 2 courses on HIV-1 replication at ENS Lyon. Go to http:// www.ens-lyon.fr/DSVT/BMC/Modules-europe/virus.html
• 2-4 March 2006, Montréal, Canada. Conference on HIV assembly at ‘Jounées Canadiennes sur le SIDA’ ( mark.wainberg@mcgill.ca).
• 31st of March. General conference on HIV at the Journées JED ‘Clermont-Ferrand’ and inteview for the newspaper LA MONTAGNE and TV FRANCE 3. ( julien.douet@univ-bpclermont.fr).
• 9-14 of August. Conference on HIV NC at the 8th international Engelhardt Conference, Moscow, RUSSIA. See web site http://science-conferences.info/ ( kissel@eimb.relarn.ru).
• 14th of September : ‘Agents infectieux, défis scientifiques et économiques’. INRP ‘francoise.jauzein@ens-lyon.fr’
• 3rd of October. Plenary conference at ENS Lyon for master students, on HIV manipulation and biotechnology issues and safety.
• 23rd of October. Pucon Chile, general conference on HIV assembly, at the PanAmerican congress in Microbiology (malopez@med.puc.cl)
• 14th of November. Café-Sida at Valence, DROME, France. ( jc.groleas@citeceramique.org).
• 4-5th of December. International conference on ‘HIV-HCV-Influenzae’ organized by JL Darlix and Eric Cohen, and the centre J Cartier. 105 attendees and 17 invited speakers from France, Switzerland, USA and Canada. Inteview for the newspaper ‘LE DEVOIR’ from Montreal. ( www.ledevoir.com; martineletarte@hotmail.com; cjc.univ-lyon2.fr/). Meeting was sponsored by ANRS and ENS (France), Sanofi-Aventis, Promega,, Qiagen (France), Réseau SIDA, Boehringer and ViroChem ( Canada). A meeting report will be published in ‘RETROVIROLOGY’ (ED. in chief KT JEANG, NIH, USA) ( www.retrovirology.com).
• Exhibition on « Camouflages » at Lyon Museum, first semester of 2006. ‘Christian.SERMET@rhone.fr’
• Caroline Goujon, Lise Rivière, Loraine Jarrosson-Wuilleme, Dominique Rigal, Jean-Luc Darlix and Andrea Cimarelli. Shared functions of VPX proteins derived from members of the HIV-2/SIVSM Lineage in the rescue of Lentiviral Infection in Human Dendritic Cells. Cold Spring Harbor 2006 Meeting on Retroviruses, Cold Spring Harbor, New York, May 23-28, 2006, pg. 111.
• The XIXth Jacques Cartier symposium on HIV and AIDS, and HCV and chronic hepatitis. ENS de Lyon, Lyon, France. 4-5th December, 2006. The role of SIV VPX in the infection of dendritic cells.
• The 2006 Meeting on Retroviruses. Cold Spring Harbor Laboratory, CSH, New York, USA, May 2006. “Late endosomal membrane proteins associated with HIV-1 virions released from EPITHELIAL AND LYMPHOCYTIC human cells.” B.Grigorov1, F.Arcanger2, P.Roingeard2, J.-L.Darlix1 and D. Muriaux1.
• The EMBO workshop on Cellular membranes and dynamics, Bilbao, Spain. June 3-7, 2006. “INTRACELLULAR ASSEMBLY AND TRAFFICKING OF HIV-1 GAG AND ITS NC MUTANTS.” D.Muriaux, D.Décimo, B.Grigorov, F.Smagulova, M.Mougel, C.Péchoux, P. Roingeard and JL. Darlix.
• The Summer ASCB 2006 meeting on The cell biology of HIV-1 and other retroviruses. Atlanta, USA, July 20-23, 2006. “HIV ASSEMBLY AND THE ROLE OF NC PROTEIN.” D.Muriaux1, B.Grigorov1, D.Décimo1, F.Smagulova2, M.Mougel2, C.Péchoux1, P. Roingeard3 and JL. Darlix..
• The XIXth Jacques Cartier symposium on HIV and AIDS, and HCV and chronic hepatitis. ENS de Lyon, Lyon, France. 4-5th December, 2006. “HIV assembly and the role of NC domain of Gag.” D.Muriaux .
• Leblanc, P., S. Alais, I. Porto-Carreiro, S. Lehmann, J. Grassi, G. Raposoand JL. Darlix. 2006. Retrovirus infection strongly enhances scrapieinfectivity release in cell culture. PRION 2006 international conference.Strategies, advances and trends towards protection of society. Torino,
• october 4-6. -Poster- http://www.newteam.it/PRION2006/
• UMR754 INRA-ENVL Université Lyon 1. Laboratoire de Rétrovirus et pathologiecomparée. Directeur JF Mornex. (Janvier 2006).
• Caroline Goujon. SIV/HIV-2 Vpx may increase lentiviral infection of human dendritic cells by counteracting a proteasome-dependent restrictive pathway. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Grigorov B, Attuil-Audenis V, Watson S, Roingeard P, Darlix JL, Muriaux D. Tetraspanins are associated with HIV- 1 virions from T-lymphocytes.

Vilarrasa :

• Lluís Bosch. Diketotetrazoles via Cu(I)-Catalyzed Cycloadditions. Oral Commun. Paris–Barcelona Workshop, 6–7 Oct. 2006.
• Joaquim Caner, HIV-1 Entry Inhibitors. Computational Studies of BMS806 Analogs and Binding Hypotheses. Oral commun. Paris–Barcelona Workshop, 6–7 Oct. 2006.
• Emilie Meddah. Synthesis of New HIV-1 Integrase Inhibitors. Oral commun. Paris–Barcelona Workshop, 6–7 Oct. 2006.
• Montserrat Terrazas. 1-Sulphonylinosines and Their Applications. Oral commun. Paris–Barcelona Workshop, 6–7 Oct. 2006.
• Lluís Bosch, Carmen Jiménez, Jaume Vilarrasa. Tetrazole- & triazole-containing Mg 2+-chelating conjugates as INIs. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Emilie Meddah, Marta Caba, Deborah Mousset, Anna M Costa, Jaume Vilarrasa. Synthesis of modified nucleosides containing Mg 2+-chelating moieties as potential INIs. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Ionela Cialicu, Joaquim Caner, Jaume Vilarrasa. N-Hydroxynucleosides mimicking the essential pharmacophore of INIs. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Vilarrasa & Esté :

• Joaquim Caner, Ionela Cialicu, Imma Clotet, José A. Esté, Jaume Farràs, Jaume Vilarrasa. Understanding the mechanism of action of BMS806 analogs. Synthesis and computational studies. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Laia Esteban, Carles Isart, Imma Clotet, Jose A Esté, Jaume Vilarrasa. Besvinyl and Tosvinyl derivatives of pyrimidine nucleosides as RTIs with alternative mechanisms of action. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Cristina Villar, Enric Llàcer, Xavier Ariza, Imma Clotet, José A Esté, Jaume Vilarrasa. Functionalized N-to-N dimeric nucleosides as polymerase or RNase-H inhibitors. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Debyser:

• De Rijck J, Vandekerckhove L, Debyser Z. The Central DNA Flap is Critically Important for HIV Replication. 13th conference on Retrovirus and Opportunistic Infections CROI, February 5-9 (2006).
• Vandekerckhove L, De Rijck J, Christ F, Poddesu B, Vercammen J, Van Maele B, Engelborghs Y, Gijsbers R, Zeger Debyser. Potent inhibition of HIV-1 replication by Over-Expression of the Integrase Binding Domain of LEDGF/p75. 13th Conference on Retrovirus and Opportunistic Infections CROI, February 5-9 (2006).
• Gijsbers R, De Rijck J, Vandekerckhove L, Christ F, Zeger Debyser. Overexpression of the LEDGF/p75 Integrase Binding Domain abrogates HIV-1 Replication. Late breaker-lecture, poster and abstract presented at Keystone Symposia Scientific Conferences on Biomedical and Life Sciences, part HIV pathogenesis. Dr. Gijsbers was awarde with a "Scholarship for HIV pathogenesis" for his presentation. March 27 – April 1, 2006.
• Batholomeeusen K, Gijsbers R, Debyser Z. Presence of a putative s/mar in the enc coding region of HIV-1 Abstract presented at Cold Spring Harbour meeting on Retroviruses (May 23-28, 2006.)
• Vandekerckhove L, De Rijck J, Rik Gijsbers, Frauke Christ, Myriam Witvrouw and Zeger Debyser. The LEDGF-integrase interaction as a new target for ART. XVI International AIDS Conference (AIDS 2006), August 13-19, Toronto. Presented at thursday, 17 August 2006 at 14h15 in the Oral Abstract Section “HIV Drug Development” and awarded with the IAS Young Investigator Award for Track A: Biology and Pathogenesis of HIV.
• Thys W, Christ F, Debyser Z. Validation of new cellular cofactors of HIV integrase and mapping of interaction sites. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Voet ARD, Vercammen J, Zhang J, Christ F, Defeyter S, Engelborghs Y, Debyser Z, De Maeyer M. Model of the HIV-1 integrase-LEDGF/p75 complex as revealed by atomic force microscopy and fluorescence  correlation spectroscopy. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Hendrix J, Vercammen J, De Rijck J, Debyser Z, Engelborghs Y. Investigation of the HIV-1 integrase interactor LEDGF/p75 with fluorescence cross-correlation spectroscopy. Oral presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Debyser and Benarous

• Busschots K, Voet A, D Maeyer M, Benarous R, Desender L, Christ F, Debyser Z. Identification of the LEDGF/P75 Binding Site in HIV-1 Integrase. Abstract presented at Cold Spring Harbour meeting on Retroviruses (May 23-28, 2006.)

Debyser and Witvrouw

• De Rijck J, Vandekerckhove L, Gijsbers R, Witvrouw M, Christ F, Debyser Z. Overexpression of the LEDGF/P75 Integrase Domain abrogates HIV-1 Replication. Abstract presented at Cold Spring Harbour meeting on Retroviruses (May 23-28, 2006.)
• Gijsbers R, Vandenhaute C, Van Laere K, Mortelmans L, Vercammen J, Engelborghs Y, Witvrouw M, Baekelandt V and Debyser Z. Viral Vector Technology for target validation and drug discovery. Abstract and poster presented at Knowledge for Growth, annual flemish biotech convention, June 16, Ghent, Belgium.
• Hombrouck A, De Rijck J, Vandekerckhove L, Christ F, Witvrouw M, Debyser Z. Virus Evolution Reveals an Exclusive Role for LEDGF/p75 in HIV. Abstract presented at the 7 th Annual Symposium on Anti Viral Drug Resistance Targets & Mechanisms. 12 – 15 November 2006, Chantilly, Virginia
• De Rijck J, Hombrouck A, Vandekerckhove L, Hendrix J, Engelborghs Y, Christ F, Witvrouw M, Debyser Z. LEDGF/p75 is the sole mediator of chromosomal tethering during HIV integration. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Hayes S, Van Remoortel B, Michiels M, Vanderveken NJ, Debyser Z, Witvrouw M. Determination of anti-HIV activity and mode of action 'is it active… and where…'. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Chimirri :

• De Luca L. 9th EU Catalyst User Group Meeting 2006 and Advanced Seminars in Catalyst. Frankfurt ( Germany) March 23-24 (2006) “Rational discovery of new anti-HIV agents”. Oral Presentation
• Chimirri A., Maria Letizia Barreca, Laura De Luca, Stefania Ferro, Anna-Maria Monforte, Pietro Monforte, Angela Rao. Palermo ( Italy) May 21-24, (2006). Molecular Modeling, Synthesis and SAR of anti-HIV agents. XII Meeting Heterocyclic Structures in Medicinal Chemistry. (pp.69-80). Oral Presentation
• De Luca L, Barreca Ml, Rao A, Monforte A.M., Chimirri A. The 16th european symposium on quantitative structure-activity relationships and molecular modeling. September 10-17 (2006). Pharmacophore modeling, docking experiments and discovery of new HIV-1 NNRTIs. (pp. 154). Poster
• De Luca L, Barreca ML, Iraci N, Ferro S, Chimirri A. TRIoH Symposium, Barcelona (Spain) December 11-12 (2006) A new 3D pharmacophore model for HIV-1 integrase strand-transfer inhibitors “ Targeting Replication and Integration of HIV”. (11) Poster

Chimirri, Witvrouw & Debyser:

• Barreca ML, Ferro S, De Luca L, Rao A, Monforte Am, Debyzeg Z, Witvrouw M, Chimirri A. XXII Congresso Nazionale della Società Chimica Italiana. Firenze (Italy) September 10-15 (2006). Molecular Modeling and synthetic approaches for new HIV-1 integrase inhibitors. (pp. 185). Poster
• Ferro S, Barreca ML, De Luca L, Rao A, Momforte AM, Visalli L, Debyser Z, Witvrouw M, Chimirri A; 6° Sigma Aldrich Young Chemists Symposium,Riccione (Italy) October 9-11 (2006) Drug Design, Innoative Strategie Sintetiche e Valutazione Biologica di Nuovi Inibitori dell’Integrasi dell’HIV-1 (P32). Poster
• Cova AM, Ferro S, Barreca ML, De Luca L, Rao A, Manforte AM, Debyser Z, Witvrouw M, Chimirri A. SCI - Convegno Congiunto delle Sezioni Sicilia e Calabria, Palermo (Italy) December 4-5 (2006) “Sintesi e SAR di inibitori dell’integrasi dell’HIV- 1 a struttura 1-benzyl-1H-indolica” .( C25). Oral Comunication
• Barreca ML, Ferro S, De Luca L, Rao A, Monforte AM, Debyser Z, Witvrouw M, Chimirri A. XXII Congresso Nazionale della Società Chimica Italiana, Firenze (Italy) September 10-15 (2006). “Molecular Modeling and synthetic approaches for new HIV- 1 integrase inhibitors” (pp. 185). Poster
• Ferro S, Barreca ML, De Luca L, Rao A, Debyser Z, Witvrouw M, Chimirri A. TRIoH Symposium, Barcelona (Spain) December 11-12 (2006) “ Solution-phase parallel synthesis of new DKA-analogues as HIV-1 integrase inhibitors ”. (12) Poster

Chimirri& Maga:

• Iraci N, Barreca ML, De Luca L, Rao A, Monforte AM, Maga G, Chimirri A. TRIoH Symposium, Barcelona (Spain) December 11-12 (2006) “Structure-based pharmacophore identificatin of new chemical scaffolds as NNRTIs”. (21) Poster
• Iraci N, De Luca L, Barreca ML, Rao A ,Maga G, Chimirri A. 6° Sigma Aldrich Young Chemists Symposium, Riccione (Italy) October 9-11 (2006) Studi Computazionali per l’Identificazione di Nuovi Inibitori Non-Nucleosidici della Trascrittasi Inversa dell’HIV-1; 6 (P44). Poster
• Iraci N, De Luca L, Barreca ML, Rao A, Maga G e Chimirri A. SCI - Convegno Congiunto delle Sezioni Sicilia e Calabria, Palermo (Italy) December 4-5 (2006) Identificazione di Nuovi Inibitori Non-Nucleosidicidella Trascrittasi Inversa dell’ HIV-1 Mediante Tecniche di Virtual screening. (P16). Poster
• Nunzio Iraci, Maria Letizia Barreca, Laura De Luca, Angela Rao, Anna Maria Monforte, Giovanni Maga, Alba Chimirri. Structure-based pharmacophore identificatin of new chemical scaffolds as NNRTIs. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Dietrich

• Antoni S, Humbert M, Brill B, Dietrich U. Mimotopes selected with broadly neutralizing IgG from long-term non-progressors induce HIV-1 neutralizing antibodies upon immunization of mice. XVI International AIDS Conference (AIDS 2006), August 13-19, Toronto.
• Humbert M, Antoni S, Landersz M, Rodes B, Soriano V, Knechten H, Staszewski S, von Laer D, Dittmar M, Dietrich U. Vaccine-relevant mimotopes selected with neutralizing IgG present in plasma from long-term non-progressors (LTNP) by phage display. XVI International AIDS Conference (AIDS 2006), August 13-19, Toronto.
• Alexandra Hüther, Tim Krüger, Christoph Königs, Xavier Dervillez, Ursula Dietrich. Generation of soluble multimeric peptides for inhibition of HIV-1 entry. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• F.G. Hermann, S. Lohrengel, M. Dittmar, U. Dietrich, D. von Laer. In-vitro selection and characterization of HIV-1 with resistance towards the membrane-bound fusion inhibitor C46. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Heinzelmann A, Crnomut D, Meyerhans A, von Briessen H, Dietrich U. APOBEC3G in infected myeloid cells as an antiviral target. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Dietrich, Divita and Mély

• Dietz J, Pustowka A, Kaur A, Koch J, Mensch S, Stein S, Grez M, Divita G, Mély Y, Schwalbe H, Dietrich U. Optimization and antiviral analysis of peptide ligands for the HIV-1 packaging signal PSI. XVI International AIDS Conference (AIDS 2006), August 13-19, Toronto.

Divita:

• Edwige Gros, Federica Simeoni, May C. Morris , G. Aldrian-Herrada, Quan N. Nguyen, Gary K. McMaster, Frederic Heitz and Gilles Divita Peptide-based nano-structure for in vivo delivery of therapeutic siRNA targeting the cell cycle regulatory protein Cyclin B1, ASCB Meeting, San Francisco, USA December 3-7 2005,
• Audrey Agopian, Natalie Bosquet-Dereuddre, Pascal Clayette, Edwige Gros, Gilles Divita. Peptide-based inhibitors of HIV-1 RT activation. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• N. Dereuddre-Bosquet 1, A. Agopian 2, R. Yousfi 1, J. Depollier 2, K. Storck 1, E. Gros 2, P. Clayette 1 and G. Divita 2 Activité anti-VIH de peptides inhibiteurs de la dimerisation de la transcriptase inverse ; 1 er Forum de l'ANRS de recherches fondamentales et cliniques sur le VIH : du patient au laboratoire, Institut Pasteur, Paris, France, 6-7 avril 2006 
• M.C. Morris 1, F. Simeoni, G. Aldrian-Herrada 1, Q Nguyen 2, G. McMaster 2, F. Heitz 1 and G. Divita 1. Peptide-based nanoparticles for in vivo delivery of siRNA targeting Cyclin B1 Meeting : Cellular delivery of therapeutic macromolecules - Cardiff , GB, August 29-31 (Invited speaker)
• L. Crombez 1, A. Charnet 1, G. Aldrian-Herrada 1, R. Brasseur 2, F. Heitz 1 and G. Divita 1. CADY: a new generation of secondary amphipathic peptide for the delivery of siRNA. Meeting : Cellular delivery of therapeutic macromolecules - Cardiff , GB, August 29-31
• Audrey Agopian1, Nathalie Bosquet-Dereuddre2, Pascal Clayette2, Edwige Gros1, Gilles Divita1.Peptide-based inhibitors of HIV-1 Reverse Transcriptase activation. XIXèmes Entretiens Jacques Cartier : Colloque "Nouvelles pandémies, les comprendre, les combattre" Les virus HIV et le SIDA, et HCV et les hépatites chroniques. Lyon, France, 4-5 décembre 2006
• Gilles Divita . Peptide-based nanoparticles for the delivery of siRNA, peptides and Bio-sensors. ASCB Meeting, San Diego, USA ; 9-12 December 2006. (Plenary session, invited speaker)
• Audrey Agopian1, Nathalie Bosquet-Dereuddre2, Edwige Gros1, Pascal Clayette2, Gudrun Aldrian-Herrada1, and Gilles Divita1. Peptide-based inhibitors of HIV-1 reverse transcription and maturation. Ist International Congress on Macromolecular Biochemistry and Genetics (CIBMG1), Gafsa, Tunisie; 12-16 Avril 2007. (Invited speaker)
• Audrey Agopian1, Nathalie Bosquet-Dereuddre2, Pascal Clayette2, Edwige Gros1, Gilles Divita1.Peptide-ba sed inhibitors of HIV-1 Reverse Transcriptase activation, 51 st Biophysical Society Annual Meeting (Baltimore Mars 2007)

Divita, Mouscadet & Witvrouw

• Gros E., Guiot E., Agopian A., Deprez E., Brochon J. C., Mouscadet J. F., Witvrouw M., Heitz F., Divita G. RT-derived peptide-based inhibitors of HIV-1 integrase reduce HIV replication in infected cells. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Gros E., Guiot E., Agopian A., Deprez E., Brochon J. C., Mouscadet J.-F., Witvrouw M., Heitz F., Divita G. Peptide based inhibitors of HIV-1 integrase reduce HIV replication in infected cells. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Engels, Debyser & Witvrouw

• Nadja V. Nikolaus, Lena Wicke, Yusuf Tanrikulu, Gisbert Schneider, Myriam Witvrouw, Zeger Debyser, Jean-François Mouscadet, Joachim W. Engels. Synthesis of arylquinolone nucleosides as novel HIV integrase inhibitors. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• Nadja V. Nikolaus, Martina M. Adams, Jan W. Bats, Myriam Witvrouw, Zeger Debyser, Joachim W. Engels. Fluoroquinolone nucleosides as inhibitors of HIV integrase. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Gottikh

• Julia Agapkina, Tatiana Prikazchikova, Marina Gottikh. The 3 rd International Scientific Conference “MedBioTech”, December 4-5, 2006, Moscow, Russia. HIV-1 Integrase: inhibition of its catalytic activity in vitro . Poster.
• Knyajanskaya E.S., Gottikh M.B.International conference of young scientists “Lomonosov 2006”. April 12-15, 2006, Moscow , Russia . Interaction of HIV and HFV integrases with palindrom substrates. Oral presentation .
• Korolev S.P., Gottikh M.B. International conference of young scientists “Lomonosov 2006”. April 12-15, 2006, Moscow , Russia . Study of HIV-1 integrase interactions with DNA using cross-linking method. Oral presentation.

Gottikh & Divita:

• Prikazchikova T.A., Volkov E.M., Romanova E.A., Mouscadet J.-F., Gilles Divita, Myriam Witvrouw, Gottikh M.B. TRIoH Scientific Symposium, December 11-12, 2006, Barselona, Spain. INHIBITION OF HIV-1 INTEGRASE CATALYTIC ACTIVITY BY MODIFIED OLIGONUCLEOTIDES. Poster.

Gottikh & Mouscadet

• Julia Agapkina, Maksim Smolov, Eric Deprez, Elena Romanova, Jean-François Mouscadet, Marina Gottikh. TRIoH Scientific Symposium, December 11-12, 2006, Barselona, Spain. 3’-Processing of HIV-1 DNA: Kinetic Study and Integrase-DNA Interactions. Poster.

Howarth & Maga

• K. F. Adebambo, S. Zanoli, M. G. Thomas, R. Cancio, G. Maga and N. M. Howarth. N 2-Benzyloxycarbonylguan-9-yl acetic acid derivatives as novel non-nucleoside reverse transcriptase inhibitors of HIV-1. Poster. TRIoH 5 th General Assembly, 11 th-12 th Dec. 2006, University of Barcelona, Spain (3 poster presentations).
• K. F. Adebambo, S. Zanoli, M. G. Thomas, R. Cancio, G. Maga and N. M. Howarth. N 2-Benzyloxycarbonylguan-9-yl acetic acid derivatives as novel non-nucleoside reverse transcriptase inhibitors of HIV-1. Poster. RSC Organic Division: 35 th Scottish Regional Meeting, 18 th Dec. 2006, Heriot-Watt University, Scotland (2 poster presentations).

Howarth & Mouscadet

• K. M. P. Collart, J.-F. Mouscadet and N. M. Howarth. 9-Aminoacridine derivatives as potential novel HIV-1 integrase inhibitors. Poster. TRIoH 5 th General Assembly, 11 th-12 th Dec. 2006, University of Barcelona, Spain (3 poster presentations).
• K. M. P. Collart, J.-F. Mouscadet and N. M. Howarth. 9-Aminoacridine derivatives as potential novel HIV-1 integrase inhibitors. Poster. RSC Organic Division: 35 th Scottish Regional Meeting, 18 th Dec. 2006, Heriot-Watt University, Scotland (2 poster presentations).

Howarth, Maga & Mouscadet

• M. G. Thomas, G. Maga, J.-F. Mouscadet and N. M. Howarth. Investigation of novel lipid-functionalised PNA monomers as potential HIV-1 non-nucleoside reverse transcriptase and/or integrase inhibitors. Poster. TRIoH 5 th General Assembly, 11 th-12 th Dec. 2006, University of Barcelona, Spain (3 poster presentations).
• K. F. Adebambo, M. G. Thomas, G. Maga, J.-F. Mouscadet and N. M. Howarth. Investigation of novel lipid-functionalised PNA monomers as potential HIV-1 non-nucleoside reverse transcriptase and/or integrase inhibitors. Poster. XVII International Roundtable on Nucleosides, Nucleotides and Nucleic Acids, 3 rd-7 th Sept. 2006, Bern, Switzerland (1 poster presentation)

Keppler

• Christine Goffinet, Nico Michel, Ina Allespach, Kerstin Ganter, Vineet N. KewalRamani, Dan R. Littman, Warner C. Greene, Mark A. Goldsmith, Oliver T. Keppler. HIV-susceptible transgenic rats: model advancement and preclinical testing of antiviral compounds targeting entry, reverse transcription and integration (WP1/3/4).
• Christine Goffinet, Nico Michel, Ina Allespach, Kerstin Ganter, Warner Greene, Mark Goldsmith, Oliver T. Keppler. Annual meeting of German Society for Virology, München, Germany, March 15-18, 2006. Talk title: Progress towards a multi-transgenic small animal model of HIV infection.
• Oliver T. Keppler: Oral presentation: Progress toward a multi-transgenic rat model for de novo infection by HIV-1, 30.03.2006, Regensburg, Germany.
• Oliver T. Keppler: Oral presentation: Progress towards an immunocompetent, multi-transgenic small animal model that can be infected with HIV, 30.06.2006, Erlangen, Germany.
• Keppler OT. Oral presentation. Progress towards an multi-transgenic small animal model for de novo infection by HIV. CSH Retrovirus Conference, May 2006
• Oliver T. Keppler: Oral presentation: Block-by-block: Progress towards a multi-transgenicrat model of HIV infection, 27.1.2006, Ulm, Germany.
• Christine Goffinet, Nico Michel, Ina Allespach, Kerstin Ganter, Vineet N. KewalRamani, Dan R. Littman, Warner C. Greene, Mark A. Goldsmith, Oliver T. Keppler. HIV-susceptible transgenic rats: model advancement and preclinical testing of antiviral compounds targeting entry, reverse transcription and integration (WP1/3/4). Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Kovacs

• L. Kovács, P. Kövér and A. Bokros (2006): 3-Deoxypentonolactones in the synthesis of chiral azetidine nucleic acid analogs. 17th International Round Table on Nucleosides, Nucleotides and Nucleic Acids, Bern, September 3-7, PO201.
• P. Pádár, J. Szolomájer, G. Ferenc, Z. Kupihár, E. Szájli and L. Kovács (2006): Preparation and examination of xanthine-containing DNA and PNA oligomers. 17th International Round Table on Nucleosides, Nucleotides and Nucleic Acids, Bern, September 3-7, PO203.
• Péter Kövér, Attila Bokros, Lajos Kovács. 3-Deoxypentonolactones in the synthesis of chiral azetidine nucleic acid Analogs. TRIoH General Assembly, December 11-12, 2006, Barcelona, poster.
• Péter Kövér, Attila Bokros, Lajos Kovács. 3-Deoxypentonolactones in the synthesis of chiral azetidine nucleic acid Analogs. 17th International Round Table on Nucleosides, Nucleotides and Nucleic Acids, Bern, September 3-7.
• L. Németh, G. Paragi, F. Bogár and L. Kovács (2006): A computational study of 3-xanthinyl derivatives in nucleobase dimers and in a PNA chain. TRIoH General Assembly, December 11-12, Barcelona, poster.
• P. Pádár, J. Szolomájer, G. Ferenc, Z. Kupihár, E. Szájli and L. Kovács (2006): Preparation and examination of xanthine-containing DNA and PNA momoners and oligomers. TRIoH General Assembly, December 11-12, Barcelona, poster.

Massa , Debyser, Witvrouw, Veljcovic

• Suvi Rajamaki, Cristina Tintori, Claudia Mugnaini, Fabrizio Manetti, Federico Corelli, Maurizio Botta, Veljko Veljcovic, Zeger Debyser, Myriam Witvrouw, Silvio Massa. Synthesis of novel potential HIV-1 integrase inhibitors. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Massa , Debyser, Witvrouw, Esté

• Cristina Tintori, Suvi Rajamäki, Fabrizio Manetti, Claudia Mugnaini, Federico Corelli, Maurizio Botta, Jose A. Esté, Zeger Debyser, Myriam Witvrouw, Silvio Massa. Structure-based virtual screening toward the identification of new HIV-1 INIs. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Massa , Maga, Esté

• Andrea Togninelli, Maddalena Alongi, Harsukh Gevariya, Claudia Mugnaini, Fabrizio Manetti, Marco Radi, Federico Corelli, José A. Esté, Giovanni Maga, Silvio Massa. Development of novel S-DABO analogues as NNRTIs. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Mély

• S.V. Avilov, E. Piémont, H. de Rocquigny, Y. Mély. Site-specific characterization of HIV-1 nucleocapsid protein binding to oligonucleotides with two potential binding sites. Lyon December.
• J. Fritz, V. Greiner, V. Shvadchak, A. S. Klymchenko, H. de Rocquigny, Y. Mély. Detection of Vpr induced apoptotic cells using 3-hydroxyflavone fluorescent derivatives. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Mély, Darlix & Gottikh:

• Sergiy V. Avilov, Nick Ramalanjaona, Chinappan Raja, Marina Gottikh, Damien Ficheux, Jean-Luc Darlix, Yves Mély. An inhibitor of HIV-1 integrase suppresses the nucleic acid chaperone function of HIV-1 nucleocapsid protein. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Mély & Langedijk:

• Volodymyr Shvadchak, Hugues de Rocquigny, Hans Langedijk, Yves Mély. Peptide inhibitors of the nucleocapsid protein of HIV. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Mouscadet :

• 7ème séminaire ANRS de recherche. Avril 2006. Données récentes sur les inhibiteurs d’intégrase. (Invited speaker)
• 27 avril 2006, Orléans, France. Conférence sur les intégrases rétrovirale.
• Convention nationale Ensemble contre le Sida. 1er juin 2006. L 'intégrase virale: nouvelle cible thérapeutique. (Invited speaker)
• Séminaires de l’IFR Châtenay, France, 11 septembre 2006. L’intégrase du VIH-1 : Nouvelle cible thérapeutique
• Meeting Synergie, 13-14 Octobre 2005 – Nouveaux inhibiteurs d’intégrase – (Invited speaker)
• Séminaire Virosem – Avignon, France. 19-22 octobre 2006 – Nouveaux inhibiteurs d’intégrase. (Invited speaker)
• 5th Pan-American congress of Microbiology, 23-26 octobre 2006, Pucon, Chili. Integrase: from Biophysics to Inhibitors (Invited speaker)
• Séminaire Vigie – Aix-en-Provence, 18-21 novembre 2006, France. Perspectives thérapeutiques et nouvelles molécules. (Invited speaker)

Restle :

• International Workshop on Discovery of Antiviral Compounds, 26 – 29 April, 2006, Lübeck, Germany - poster
• Veldhoen S, Laufer SD, Trampe A, Restle T. Cellular delivery of therapeutic macromolecules - A Biochemical Society Focused Meeting in association with the Royal Society of Chemistry, the Royal Pharmaceutical Society of Great Britain and the Academy of Pharmaceutical Sciences, 29 - 31 August, 2006, Cardiff, UK - poster
• RNA Chemistry meets Biology, 29-30 September, 2006, Lund, Sweden - poster
• 4th Meeting of the GBM Study Section “RNA-Biochemistry”, 12 – 15 October, 2006, Kassel, Germany – poster.

Restle & Massa :

• Dina Grohmann, Valentina Corradi, Florian Horenkamp, Sandra D. Laufer, Fabrizio Manetti, Maurizio Botta, Tobias Restle. Small molecule inhibitors targeting HIV-1 RT dimerization. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Robina

• Y. Vera-Ayoso, A. T. Carmona, A.  Ferrali, F. Cabrera, A. J. Moreno-Vargas, L. Molina, F. Cabrera, I. Robina. Towards new indole derivatives as potential anti-HIV agents. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.
• A. Szczepanska, A. T. Carmona, J. L. Espartero, L. Molina, A. J. Moreno-Vargas, H. Li, Y. Guan, G. K. Lewis, L. X. Wang, I. Robina. Synthesis and anti-HIV activity of trivalent CD4-mimetic miniproteins. Structural and conformational analysis of their templates. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Veljkovic

• Glisic S., Veljkovic N., Veljkovic V. EIIP criterion for selection of natural compounds with anti-HIV activity. TRIoH Scientific Symposium_ Barcelona (2006) - poster
• Rajamaki S., Tintori C., Mugnaini C., Manetti F., Corelli F., Botta M., Veljkovic V., Debyser Z., Witrouw M., Massa S. Synthesis of novel potential HIV-1 integrase inhibitors. TRIoH Scientific Symposium_ Barcelona (2006) – poster

Villalain

• The membrane-active regions of the HIV gp41 env glycoprotein full sequence, 50th Biophysical Society Meeting, Salt Lake City, Utah, USA, 2006
• XXIX SPANISH BIOCHEMICAL AND MOLECULAR BIOLOGY SOCIETY MEETING. Elche, September 2006. FOUR COMMUNICATIONS.
• VI IBERO-AMERICAN CONGRESS OF BIOPHYSICS. Madrid, Octobver, 2006. TWO COMMUNICATIONS.
• 13TH INTERNATIONAL MEETING ON HEPATITIS C VIRUS AND RELATED VIRUSES. Cairns, August, 2006. ONE COMMUNICATION.
• Miguel Moreno, José Villalain. Searching for peptide inhibitors of gp41 mediated HIV-entry. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

Vogel

• Synthesis of conduramines epoxides: new alpha-glucosidase inhibitors. Robert Lysek, Catherine Schütz, Pierre Vogel. Poster presentation on TRIoH Symposium December 11-12, 2006, Barcelona.

2007

Debyser: CROI, CSH.

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3. OTHER INITIATIVES

2004

Witvrouw:

Press releases, beginning of 2005 in Belgian press (TV, radio, national news papers, university press)

Esté:

• The WP leader (Partner 2, Esté) is coordinating a recently formed “Platform for HIV-drug screening” as part of the Spanish AIDS Research Network
• The WP leader (Partner 2, Esté) is local organizers of the 18th International Conference on Antiviral Research to be held in Barcelona, April 2005.

Chimirri:

• Organization of the TRIoH consortium meeting in Messina

Darlix:

• See TV and radio interviews. HIV presentations to teachers and students.

Dietrich:

• Invitations of TRIoH-members to the Georg-Speyer-Haus as seminar speakers: Zeger Debyser: “LEDGF, a novel cellular co-factor of HIV integration”
• Organization of a 2 days symposium at Georg-Speyer-Haus „Peptides as drugs: problems and promise“ by Ursula Dietrich;
• TRIoH speakers include Gilles Divita and Ursula Dietrich
• Scientific meetings with TRIoH partners Yves Mély and José Villalain at Georg-Speyer-Haus
• A student of José Villalain (Miguel Moreno) spent several weaks at the Georg-Speyer-Haus to perform a collaborative work within TRIoH.

Mouscadet:

• A one-week exchange between our group and partner 11 was organized to discuss progress on anti-IN oligonucleotide studies.

Restle:

• teaching activities University of Luebeck - presentation of certain aspects of the project to students

Vilarrasa:

• A secondment of M Terrazas to the University of Siena, Partner 17, has been agreed for April-June 2005. Participation in the 18th International Conference on Antiviral Research ( Barcelona, April 10–14, 2005).

Mouscadet

Two students from partner 11 spend respectively 1 mo and 2,5 mo as part of common project.

2005

Witvrouw and Debyser:

• We have initiated contacts with biotech and pharmaceutical companies that are interested in the developments and the tools that we have generated for anti-HIV integrase (WP4) and cellular co-factors (WP5) research. Talks and collaboration have been initiated for the development of anti-HIV compounds.

Esté:

• We have initiated contacts with biotech and pharmaceutical companies that may be interested in the developments and the tools that we have generated for anti-HIV entry research. Talks and collaboration have been initiated for the development of anti-HIV compounds.
• Esté is actively participating in the International Society for Antiviral Research (ISAR) as member of the Conference Committee and providing service to the society.
• Esté has been appointed to the Editorial Board of the journal Antiviral Research
• Esté has been appointed Editorial Consultant to of the journal The Lancet
• Esté has been appointed to the Editorial Board of a new journal Future Virology.

Vilarrasa :

• Dr Ana T Carmona, from Sevilla (Partner 21), spent the first week of June in Barcelona (Partner 4), as a part of the Training Program, getting knowledge of MacroModel 8.5 (conformational analysis program) and docking programs, as well as of their application to the design and improvement of the glycopeptides and peptoids are being synthesizing in Sevilla.
• Montserrat Terrazas (from Partner 4) spent the months of April, May and June in Siena (Partner 17), as a part of the TRIoH Training Program, collaborating with the project of Partner 17 and participating, with full registration, in the Fifth European Workshop in Drug Design held overthere the first week of June.
• Oriol Pineda and Joaquim Caner (from Partner 4) went to Sevilla (Partner 21) to help with the installation and applications of MacroModel 8.5. They spent overthere from October 24 to October 27.
• Pineda and J Caner participated in the Workshop held in Bonn on November 9–11, regarding the FlexX 2 computer program, the latest release of BioSolveit GmbH for the prediction of protein–ligand complexes (molecular docking).
• Pineda and J Caner participated in the TRIoH Meeting held in Pavia (30 Nov–2 Dec) where the first one delivered a talk regarding the Training Program about Molecular Modeling and Docking and the second one presented his computational results on the interaction of BMS analogues with gp120.
• Registration of the group leader to the 8th International Conference on Antiviral Research held in Barcelona, April 11–14, facilitated the assistance of all the TRIoH Barcelona members to the poster sessions.
• Montserrat Terrazas, Joaquim Caner and Prof. Vilarrasa participated in the RANN 5 (Reunión de Acidos Nucleicos y Nucleosidos–5) held in the University of Alcalá (Madrid) on October 28 and 29, where the first two presented their (non-confidential) results.
• All the TRIoH Barcelona members participated in the Workshop of the Network “Synthesis of Natural Products and Enantiopure Drugs”, held in Barcelona on September 16, in which apart from 20 short communications, there was a plenary lecture of Prof. Dieter Seebach (ETH, Zurich) and two talks delivered by the research managers of two pharma companies (Laboratoris Almirall & Lab. Esteve Química).
• All the TRIoH Barcelona members participated in the Course on Medicinal Chemistry (“Drug discovery case studies” / “Structure-based & computer-aided design” / “Virtual screening – the road to success”) delivered by Prof. Hugo Kubinyi in Barcelona on November 4. Participants were: (i) Prof. Vilarrasa, Oriol Pineda (M) (management). (ii) Dr. Farràs, Joaquim Caner & Ionela Cialicu (F), members of WP1. (iii) Dr. Ariza, Montserrat Terrazas (F), Laia Esteban (F) & Cristina Villar (F), of WP3. (iv) Dra. Costa, Lluís Bosch (M), Emilie Meddah (F) & Gorka Etxebarria (M), of WP4. [TRIoH-funded people are highlighted.]

Chimirri:

• Seminars for PhD students at the University of Messina - presentation of certain aspects and non confidential results of the TRIoH project
• Internal seminars for PhD students at the University of Messina - presentation of certain aspects and non confidential results of the TRIoH project.
• A member of my group (ML Barreca) spent one week at Siena ( Italy) in the laboratory of partner 17 to discuss progress on anti-HIV agents.
• A member of my group (A. Rao) spent one week at Siena ( Italy) in the laboratory of partner 17 to acquire new synthetic methods.

Dietrich:

• Scientific meetings with collaborating TRIoH partners (Mély, Engels, Divita)
• Organization of the TRIoH consortium meeting in Frankfurt

Engels:

• A student from Frankfurt, Lena Wicke, spent 3 months in N. Howarth group in Edinburgh to perform PNA studies.

Gottikh:

• teaching activities in the Moscow State University - presentation of certain aspects of the project to students

Keppler:

• A Ph.D. student (Christine Goffinet) spent one week at the KU Leuven, Belgium in the laboratories of Zeger Debyser and Myriam Witvrouw to acquire Q-PCR skills (collaborative work within TRIoH).
• Co-organizer of internal and external seminar series of SFB 544 (“control of tropical infectious diseases”) in Heidelberg, Germany.
• Teaching activities for Medical students at the University of Heidelberg - presentation of certain aspects of the project to students.

Mely:

• Scientific meetings with TRIoH partners: JL Darlix and JC Brochon at Strasbourg

Mouscadet:

• Two students from partner 11 spend respectively 1 mo and 2,5 mo as part of common project.

Villalain:

• MR.Moreno, PhD student from Villalaín´s lab, visited the laboratory of Dr. U. Dietrich at the end of 2004 to work on Phage Display and SPR of gp41-derived peptides.

2006

Witvrouw:

• Training activities: Christina Tintori (PhD student from P17) spend April – May 2006 in Leuven.

Witvrouw and Debyser

• We have initiated contacts with biotech and pharmaceutical companies that are interested in the developments and the tools that we have generated for anti-HIV integrase (WP4) and cellular co-factors (WP5) research. Talks and collaboration have been initiated for the development of anti-HIV compounds.

Debyser:

• Training activities: Martine Michiels (head lab technician) stayed from 10-22 september 2006 in Badelona, Spain at Esté’s lab to learn how to isolate monocytes and to let them differentiate into macrofages.

Vilarrasa :

• Training activities. Two members (Dr. A. Rao and doctorate student A. Grimaldi) of Chimirri group spent from 4 to 7 October 2006 at Barcelona, in the laboratory of Partner 4, where they also participated in the Paris-Barcelona workshop held in the Faculty of Chemistry.
• Training activities. Two PhD students (C. Villar and L. Esteban) from Partner 4 spent one week (2–7 Nov. 2006) at the University of Messina ( Italy) to get acquaintance of the synthetic methods utilitzed overthere aimed at starting a collaboration in the near future.
• Training activities. Dr. Anna Ma. Costa, a researcher involved in the project from the very beginning, participated in the Gaussian Workshop held on 6–9 June 2006.

Chimirri :

• Two Ph.D. students (C. Villar and L. Esteban) from partner 4 (Vilarrasa) spent one week at the University of Messina (Italy) to acquire synthetic methods.
• Two members (A. Rao and A. Grimaldi) of Chimirri group spent one week at Barcelona (Spain) in the laboratory of partner 4 (Vilarrasa) where also participated in the Paris-Barcelona workshop held in the Faculty of Chemitry (University of Barcelona).
• Seminars for PhD students at the University of Messina - presentation of certain aspects and non confidential results of the TRIoH project.
• Talks and collaborations have been initiated for the development of anti-HIV compounds.
• Philippe Cotelle spent some days in the Chimirri group at University of Messina for the design of new IN strand-transfer inhibitors.
• Seminar for PhD students of University of Messina by Philippe Cotelle on “Total synthesis of some natural polyphenols and their anti-HIV properties”

Engels :

• based on a seminar given in Frankfurt on HIV Integrase inhibitors we got the cheminformatics group of Prof. Schneider involved in drug design, in the person of Y. Tanrikulu

Gottikh :

• One post-doc and one PhD student spent 3 and 2 months respectively in J-F Mouscadet lab and one student spent one month in G.Divita lab as part of the project.

Maga:

• In the context of establishing connnections with developing countries and of translating/disseminating the knowledge, Prof. Bechan Sharma, PhD, Professor of Biochemistry at the University of Allahabad (India) spent a two months sabbatical to conduct experiments related to the Project in the laboratory of P16 (June-July 2006).

Keppler :

• We have initiated contacts with biotech and pharmaceutical companies that are interested to screen anitviral compounds in the transgenic rat model. Contracts for two pilot studies have been formalised.

Mouscadet:

• One Polish scientist was welcomed for 15 days for training in integrase activity testing.
• A Russian student has started a joint PhD shared between JF Mouscadet’s and M. Gottikh’s groups.
• Organization of the TRIoH consortium meeting in Paris, June 2006.
• Eric Deprez & JF Mouscadet participated to the setting up of the Erasmus Mondus Master program “MONABIPHOT” in which data concerning biophysics of Integrase were presented. E. deprez is responsible for the biophysics module.
• JF Mouscadet participated to the scientific comitees of the 2 nd Workshop on entry inhibitors and novel therapeutic classes. Montpellier 2007 and the 3 rd Dominique Dormont Internationl Conference on host- pathogens interactions, 2006, Bordeaux France.
• JF Mouscadet participated to the XVI International AIDS Conference board of abstract reviewers ( Toronto, 2006).

Restle : teaching activities University of Luebeck - presentation of certain aspects of the project to students

Villalain :

• General lectures on HIV, membrane fusion and mechanism for under and post-graduate students, both at the University "Miguel Hernández" and at the University of Murcia.
• Exhibition of posters concerning TrioH research at the "Week of Science" in the University "Miguel Hernández", October, 2006.

2007

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4. PUBLIC EFFORTS

2004

Witvrouw:

• Press releases, beginning of 2005 in Belgian press (TV, radio, national news papers, university press) - see list.
• Website Consortium: http://www.kuleuven.ac.be/molvirgen/projects/trioh/index.htm

Esté:

• The WP Leader (Partner 2, Esté) has been invited speak at the European Drug Discovery Technology Meeting, London, UK, 2005. We hope to describe the projects objectives and raise awareness about our goals

Darlix:

Interviews on Radio and public TV’s

• TLM (Télévision Lyon Métropole) 29th January 2004.
• Cité Campus interview by Sandry Vicente ( svicente@tlm.fr)
• France 3. 22nd April 2004. Mid day magazine : 12-14 :00 am.
• Interview by Mr. Bette (0033 4 78 14 63 24).
• M6. 23rd April 2004. M6 magazine at 11 :00 am (Sophie 033 4 78 37 16 16)
• France 5/ARTE. 1st December 2004. Magazine de la santé sur le SIDA, 13-14 :00 am. ( assistant.mediat@francetv.fr)

General conferences on HIV and AIDS to adults (virus, viral infection, prevention and HAART

• Café-Science "SIDA : fin de l'indifférence ?", 4th december 2003, Annonay, France
• (3 :30 pm scholars) (8 : 30 pm MJC Annonay for adults). Organizer JC Groléas.
• Ciné Sciences, Cinéma Le Pestel Die, France. 27th April 2004. 2 pm to 10 pm (teenagers and adults). Organizer Jean-Christophe Groléas (jc.groleas@citeceramique.org ).
• CCSTI de la Drôme BP 58, 26241 St Vallier Cédex Tél. 033 4 75 03 27 52.
• Promega General conference on the origins of HIV, Lyon, France. 27th May 2004. Organized by Promega France (isabelle.baginski@promega.com ).
• Faculty of Medicine, Tours, France. 3rd of June 2004 (Pr. Ph Roingeard: roingeard@med.univ-tours.fr ).
• Institut de recherche et développement (IRD), 9th and 10th June 2004; Banuyls, FRANCE Organizer Dr. Frederique LEROUX : ( Frederique.Leroux@ifremer.fr ).
• Faculty of Medicine of SOFIA, BULGARIA ; 30th of June 2004. Organizer Prof. Radka Argirova ( argirovaradka@hotmail.com ).
• Université Européenne d’été, Toulouse, FRANCE, 12th July 2004. Organizer Justin TEISSIE : ( Justin.Teissie@ipbs.fr .).
• Université d’été des jeunes chercheurs sur le SIDA, Marseille, France. 21st, 22nd September 2004. Frédéric Mattéi AIDES-RAM, Marseille. ( mattei.frederic@wanadoo.fr ).
• Lyon, conference on HIV in Molecular Medicine. 26th November 2004. 10-12 :00 am. Organizer Prof. G. Brun ( Gilbert.Brun@ens-lyon.fr ).

Dietrich:

• General HIV lectures (not especially related to TRIoH) are given for highschool boys and girls as well as their teachers once a year at the Georg-Speyer-Haus, organized by Ursula Dietrich

Gottikh:

• One visit of M. Gottikh to the group of J-F. Mouscadet (P19) in order to discuss the results concerning the inhibitory activity of the oligonucleotide conjugates prepared and to select conjugates which will be tested in PIC assay.

Restle:

• exhibition of posters concerning aspects of the TRIoH project

Veljkovic:

• Press conference concerning TRIoH – World AIDS day (12/1/2004) –International press center, Belgrade, Serbia

Villalain:

• Several presentations to the students of Biochemistry of the University “Miguel Hernández”.

 

2005

Chimirri:

• Oral and/or posters presentations during national and international conferences concerning aspects and non confidential results of the TRIoH project.

Dietrich:

• General HIV lectures (not especially related to TRIoH) are given for highschool boys and girls as well as their teachers once a year at the Georg-Speyer-Haus, organized by Ursula Dietrich

Mouscadet

• Interview on HIV research on French television (TF1 channel), April 2.

Restle:

• Teaching activities University of Luebeck - presentation of certain aspects of the project to students

Villalain:

• Along the year, several HIV seminars are given for the students at the University "Miguel Hernández"

 

2006

Witvrouw

'Wetenschapsweek': week of science, initiative to promote science edution with interactive session on science topics. Presentation on HIV and the Blood. Seminar plus lab session on 20 october 2006 in Kortrijk.

Witvrouw & Debyser:

Disseminiation in the European Parliament by publication in Parliament Magazine. Successful FP6 Integrated Project TRIoH results in new targets and new drugs for anti-HIV therapy. Parliament Magazine 235/236 (11/12/06) p31.

In July-August 2006 we did an attempt to get our HIV-research in the local press. This because of one of our researchers got an award at the Toronto Congress. The Toronto congress however got little attettion in the local press, so this attempt succeded only with a short announcement in one paper (De Tijd, 16 August 2006) and an interview with Linos Vandekerckhove in “De Campus Krant”, the magazine of the KULeuven. http://www.kuleuven.be/ck/2006_07/01/ck18-01-hiv.php.

 

Mouscadet:

• Seminars on HIV and HIV pharmacology were given to students in Master program from Universities Paris XI, Paris V, and form the Following schools: Institut for Biomedical Research, Ecole Supérieure de Biotechnologie Appliquée.
• JF Mouscadet participated to a meeting with AIDS patients organized by the French association “Act-Up” to inform patients about novel molecules (Mai 2006).

 

Chimirri :

• Oral communications, invited lectures and posters were presented during national and international conferences concerning aspects and non confidential results of the TRIoH project.
• L ectures about HIV agents have been given for students of Faculty of Pharmacy of Messina University

 

Engels :

• During the Night of Science in Frankfurt 2006 a lecture on HIV inhibition and drug design was given

 

Gottikh :

• Teaching activities in the Moscow State University - presentation of certain aspects of the project to students.

Kovacs :

• Oral communications, invited lectures and posters were presented during national and international conferences concerning the results of the TRIoH project.
• L. Kovács (2006): The synthesis of chiral heterocycles from carbohydrates (in Hungarian). Bruckner room lectures, Budapest, February 24, lecture.
• L. Németh, G. Paragi, F. Bogár and L. Kovács (2006): The study of 3-xanthinyl derivatives in dimer systems and in PNA skeleton (in Hungarian). Annual Meeting of the Chemometry and Molecular Modelling Workshop, Szeged, April 8, lecture.
• A. Bokros, P. Pádár, P. Forgó, Z. Kele and Kovacs (2006): Chiral, nitrogen-containing heterocycles from carbohydrates (in Hungarian). Annual Meeting of the Hungarian Carbohydrate Workshop, Mátrafüred, June 1, lecture.
• L. Kovács (2006): Summary of results (WP1, WP3 and WP4). TRIoH General Assembly, Paris, June 6-7, lecture
• A. Bokros, P. Forgó, Z. Kele and Kovacs (2006): The transformation of bicyclic isoxazolidines into potential glycosidase inibitors (in Hungarian). Annual Meeting of the Hungarian Nucleotide Chemistry Workshop, Debrecen, September 21, lecture.
• A. Bokros, P. Pádár, P. Forgó, Z. Kele and L. Kovács (2006): Synthesis and transformation of bicyclic isoxazolidines (in Hungarian). 29th Chemical Presentation Days, Szeged, October 30-31, lecture.

 

 

 

 

Copyright © Katholieke Universiteit Leuven | reacties op de inhoud: Peter Bogaerts
Realisatie: Bruno Hoste| Laatste wijziging: 8-05-2007
URL: http://www.kuleuven.be/molvirgen/projects/trioh/newpublicationtr.htm