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- Biochemical analysis of allergen extracts - Quantification of cytokines in biological fluids: interleukin 2 interleukin 4, Tumor Necrosis Factor (ELISA), interleukin 6, interleukin 10, interferon-þ, interleukin 5, interleukin 12 - Screening of monoclonal antibody reactivity with cell surface antigens on FACS - Determination of composition, biological activity and shelf life of allergenic extracts. - Preservation and stabilization of allergenic extracts. - Extraction of allergenic proteins from biological substrates.
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| Description expertise techniques : |
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- Isoelectric focusing and blotting techniques - FACS analysis of cell surface fluorescence - Chromatography - Lymphocyte functional analysis (profileration, activation marker expression, cytokine production...) - Lymphocyte and subpopulation isolation from blood or tissues - Immunoblot and quantitative IgE binding inhibition techniques. - Quantification of cytokines in biological fluids and culture super - Animal models of autoimmunity (inflammatory bowel disease, arthritis and encephalicitis) - Animal models of allergy. - FACS sorting
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| Description expertise users : |
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- Companies specialized in the preparation of allergen extracts - Cell biology - Monoclonal antibody production - Producers immunological drugs - Immunopharmacological drugs
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Research team to which CEUPPENS JAN belongs. Expert number:X3696
Nederlands |