SCIL > Core section > Q-PCR Core
Q-PCR Core
PURPOSE:
The facility provides real-time PCR chemistries consultation, experimental design consultation, assistance in data analysis and troubleshooting.
FACILITIES:
The facility is located in SCIL on the 8th floor of O&N1 on the Gasthuisberg Campus of the K U Leuven. The facility operates two Mastercycler? ep realplex4, capable of absolute and relative quantification (gene expression analysis), SNP analysis, endpoint detection (+/- assays, gene ID) and melting curve analysis. Detection of all dyes most commonly used for real-time PCR is possible (e.g. SYBR Green, FAM, VIC, JOE, NED, TAMRA; not possible: Cy3, Cy5).
SERVICES:
The facility does not setup reactions for Investigators, but support is available upon request. Machine operations are self served for experienced users from individual labs. Training sessions are highly recommended for first time users. Please contact the Core Facility to setup an appointment.
PRICES:
Training: no cost
Per reaction: SCIL members €20.00/run; non-members: €40.00/run
CONTACT & APPOINTMENTS:
Vicky Raets; 016 330296; vicky.raets@med.kuleuven.be
